Characterization of Immunophenotype and Differentiation Potential of Human Bone Marrow Mesenchymal Stromal Cells after Cryopreservation

Authors

  • Yu. A. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. G. Skorobogatova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Volkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, mesenchymal stromal cells, human bone marrow, adipogenic and osteogenic differentiation, immunophenotype

Abstract

Stromal cells derived from adult human bone marrow (BM) after in vitro expansion demonstrate a specific immunophenotype and ability to multilineage differentiation, inherent to mecenchymal stromal cells (MSCs)m. It was established that cryopreservation of human BM MSCs by two-step freezing under protection of 10% DMSO enables to preserve their immunophenotype and ability to induced osteogenic and adipogenic differentiation in vitro.

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Published

2011-01-10

How to Cite

Petrenko, Y. A., Skorobogatova, N. G., Volkova, N. A., & Petrenko, A. Y. (2011). Characterization of Immunophenotype and Differentiation Potential of Human Bone Marrow Mesenchymal Stromal Cells after Cryopreservation. Problems of Cryobiology and Cryomedicine, 20(4), 436–442. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/172

Issue

Vol. 20 No. 4 (2010): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 Yu. A. Petrenko, N. G. Skorobogatova, N. A. Volkova, A. Yu. Petrenko

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