Comparative Evaluation of Wound Healing Effect of Cord Blood Low Molecular Weight Fraction (Below 5 kDa) and Actovegin Drug in Skin Cryolesions
DOI:
https://doi.org/10.15407/cryo31.02.116Keywords:
cryoablation, cord blood, Actovegin, rats, wound surfaceAbstract
This paper presents the results of comparative studies of the impact of the low molecular weight (below 5 kDa) cattle cord blood fraction (CBF) and the comparator drug Actovegin on tissue regeneration after skin cryoablation in rats. Here, a local contact cryoexposure was shown to result not only in destruction of all skin layers, but secondary ischemic damage to deeper and adjacent tissues as well. The use of an injection solution of CBF as a therapy for simulated cryolesion proved its pronounced wound healing effect, manifested in accelerated reparation, improved trophicity in an injured area due to hypervascularization and prevention of sclerotic processes. The introduction of CBF and Actovegin to rats accelerated the normalization of clinical blood parameters (RBC and WBC counts, alkaline phosphatase activity) in shorter terms as compared with the control. The obtained effect may be compared with Actovegin action.
Probl Cryobiol Cryomed 2021; 31(2): 116–126
References
Amankulova SJ. [The use of cryodestruction for the treatment of Verruca plantaris]. Vestnik AGIUV. 2016; (1): 57-60. Russian.
Bilgic S, Ozkan H, Ozenc S, et al. Treating frostbite. Can Fam Physician. 2008; 54(3): 361-3.
Buchet R, Millán JL, Magne D. Multisystemic functions of alkaline phosphatases. Phosphatase Modulators. 2013; 1053: 27-51. CrossRef
Cranwell WC, Sinclair R. Optimising cryosurgery technique. Aust Fam Physician. 2017; 46(5): 270-4. PubMed
Dyomin YuA, Gulevsky AK, Seydaliyeva ZA, et al. [Influence of a low-molecular fraction (below 5 kDa) from cord blood as an eye gel component on cornea regeneration after mechanical damage]. Svit Medytsyny i Biolohii. 2013; (3): 7-11. Russian.
Fisher SR, Rigby JH, Mettler JA, et al. The effectiveness of photobiomodulation therapy versus cryotherapy for skeletal muscle recovery: a critically appraised topic. J Sport Rehabil. 2019; 28(5): 526-31. CrossRef
Gage AA, Baust JM, Baust JG. Experimental cryosurgery investigations in vivo. Cryobiology. 2009; 59(3): 229-43. CrossRef
Gulevsky AK, Abakumova YS, Moiseyeva NN, et al. Influence of cord blood fraction (below 5 kDa) on reparative processes during subchronic ulcerative gastropathy. Ulcers [Internet]. 2011 Sep 05 [cited 2018 Feb 01]; 2011: Article ID 214124. Available from: https://www.hindawi.com/journals/ulcers/2011/214124/ CrossRef
Gulevsky AK, Akhatova YuS, Sysoev АА, et al. V. Energy metabolism of packed white cells after cryopreservation and rehabilitation in a medium containing a cord blood low molecular fraction. Biotechnologia Acta. 2015; (6): 63-70. CrossRef
Gulevsky OK, Akhatova YuS, Sysoev АА, et al. [Stimulating effect of a low-molecular fraction from cord blood on the energy metabolism in leukocytes]. Dopovidi NAN Ukrainy. 2014; (7): 152-7. Russian. CrossRef
Gulevsky OK, Moiseeva NM, Abakumova OS, Shcheniavsky II, Nikolchenko AY, Gorina OL, inventors; Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, assignee. [A method of obtaining a low-molecular fraction from the blood of cattle]. Ukraine patent UA 69652. 2012 May 10. Ukraine
Gulevsky OK, Schenyavsky II. Antihypoxant activity of low molecular weight fraction bovine blood cryohemolysate at different stages of ontogenesis. Probl Cryobiol Cryomed. 2017; 27(1): 41-50. CrossRef
Korzhevskij DE, Gilyarov AV. [Fundamentals of histological technique]. St. Petersburg: SpecLit, 2015. 807 p. Russian.
Kovalov GA, Ischenko IO, Naumova OV, et al. [Morphological characteristics of wounds caused by cryodestruction]. Nauchnye vedomosti BelGU. Mediczina. Farmacziya. 2015; (21): 107-14. Russian.
Lazarenko VA, Artiushkova EB, Mishustin VN, et al. [Combined pharmacological correction of experimental frostbite]. Vestnik eksperimentalnoj i klinicheskoj khirurgii. 2013; 6(1): 45-9. Russian.
Mikhin IV, Kukhtenko YuV. [Burns and frostbite]. Volgograd: VolgGMU, 2012. 88 p. Russian.
Millán JL. Alkaline phosphatases: structure, substrate specifi city and functional relatedness to other members of a large superfamily of enzymes. Purinergic Signalling. 2006; (2): 335-41. CrossRef
Nadarajah S, Ariyagunarajah R, Jong ED. Cryotherapy: not as cool as it seems. J Physiol. 2018; 596(4): 561-2. CrossRef
Okunev NA, Okuneva AI, Vlasov AP, et al. [Local cryoablation hemangiomas in children]. Vestnik eksperimentalnoj i klinicheskoj khirurgii. 2013; 8(2): 195-201. Russian.
Pavlov AV, Maskin SS, Igolkina LA. [Local cryotherapy in treatment of purulent skin diseases and subcutaneous tissue]. Journal of Volgograd State Medical University. 2018; (1): 3-9. Russian. CrossRef
Point M, Guilhem G, Hug F, et al. Cryotherapy induces an increase in muscle stiffness. Scand J Med Sci Sports. 2018; 28(1): 260-6. CrossRef
Shafranov VV, Borhunova EN, Kostylev MA, et al. [The mechanism of destruction of biological tissue at a local cryodestruction]. Vestnik RAEN. 2012; 12(1): 68-77. Russian.
Shchenyavsky II. [Influence of the fraction below 5 kDa, extracted from the calf cord blood, on lactate and pyruvate contents in the rat liver after glucose loading]. Vestnik problem biologii i mediciny. 2009; (4): 118-20. Russian.
Shchenyavsky II. [Influence of the fraction under 5 kDa from the cattle cord blood on glucose, lactate and pyruvate contents in burn wound tissues]. Eksperymentalna ta klinichna fiziolohiia i biokhimiia. 2010; 1(49): 50-3. Ukrainian.
Sheiko EA. [Hemangiomas in young children]. Mezhdunarodnyj zhurnal prikladnykh i fundamentalnykh issledovanij. 2015; (4): 222-8. Russian.
Shubich MG, Nagoyev BS. [Alkaline phosphatase in health and disease]. Moscow: Medicina, 1980. 224 p. Russian.
Tits NU. [Encyclopedia of clinical laboratory tests]. Moscow: Labinform, 1997. 960 p. Russian.
Zimmerman E, Crawford P. Cutaneous cryosurgery. Am Fam Physician. 2012 ;86(12):1118-24. PubMed
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
