Endocrine Function of Ovarian Tissue Grafts After Normo- and Hypothermic Storage

Authors

  • Viktoria V. Kiroshka Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Yulia O. Bozhkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Ahmed A. Gavas V.N. Karazin Kharkiv National University, Kharkiv
  • Iryna A. Rula Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Tetyana P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv https://orcid.org/0000-0002-5258-3741
  • Galyna O. Semko Kharkiv National Medical University, Kharkiv
  • Volodymyr O. Pyatikop Kharkiv National Medical University, Kharkiv

DOI:

https://doi.org/10.15407/cryo31.04.326

Keywords:

hypothermic storage, normothermic storage, morphological characteristics, follicles, sex hormones, mannitol-containing solution

Abstract

Morphological characteristics and function of ovarian tissue after 2–120 hrs of storage at 4 and 37°C in incubation media with different composition (mannitol-containing solution (MСS) and phosphate buffered saline (PBS)) were investigated. The function of ovarian tissue after normo- and hypothermic storage was studied by heterotopic transplantation to recipient animals simultaneously with bilateral ovariectomy. After 2-hour storage at 37ºC and 24-hour one at 4ºC in all the studied media, the estradiol and progesterone levels in plasma of recipient animals were experimentally proved to be 18.2–27.5 and 13.5–18.5 ng/ml, respectively, (after fresh tissue transplantation these values for estradiol and progesterone made (28.1 ± 3.6) pg/ml and (19.1 ± 2.8) ng/ml, respectively). Prolongation of tissue storage time at 37ºC increased the number of degenerative follicles, augmented TBARS concentration and reduced sex hormone levels after transplantation using both MCS and PBS. A protective effect of MCS only, manifested in endocrine function restoration in recipient animals after transplantation ((13.5 ± 2.5) pg/ml of estradiol) was found after 48 hrs of ovarian tissue hypothermic storage. If PBS was used, the estradiol level was within the indices for ovariectomized animals ((7.1 ± 1.5 (pg/ml)).

 

Probl Cryobiol Cryomed 2021; 31(4): 326–342

Author Biographies

Viktoria V. Kiroshka , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

Department of Cryoendocrinology 

Yulia O. Bozhkova , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology 

Iryna A. Rula , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology 

Tetyana P. Bondarenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv

Department of Cryoendocrinology 

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Published

2021-12-24

How to Cite

Kiroshka , V., Bozhkova , Y., Gavas , A., Rula , I., Bondarenko , T., Semko , G., & Pyatikop, V. (2021). Endocrine Function of Ovarian Tissue Grafts After Normo- and Hypothermic Storage. Problems of Cryobiology and Cryomedicine, 31(4), 326–342. https://doi.org/10.15407/cryo31.04.326

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Section

Theoretical and Experimental Cryobiology