Opportunities of Electron Microscopy When Solving Cryobiological Tasks. Retrospective Analysis
DOI:
https://doi.org/10.15407/cryo32.01.003Keywords:
electron microscopy, freezing-fracture, freeze-substitution, cryofixation, crystal formation, erythrocytes, membraneAbstract
The history of the development and use of low-temperature electron microscopic methods of freeze-fracture, freeze-substitution and others in cryobiological research at the Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine is presented in this article. These methods’ possibilities in studying the processes of crystal formation in cryoprotectant solutions, cell suspensions, and tissues under various freezing conditions are demonstrated. Some results of the analysis of ultrastructural changes in biological systems of various organization levels, at different stages of ontogenesis, under the influence of cooling in a wide temperature range (from 37 to –196°C) are presented. The use of a high resolution electron microscopic method in combination with an accessory technical equipment and some methodological techniques allowed to obtain fundamental results important for cryobiology on ice crystals formation and localization in the intracellular volume, the temperature-dependent transmembrane proteins redistribution, changes in the ultrastructure of erythrocytes and their membranes during hypothermic storage.
Probl Cryobiol Cryomed 2022; 32(1): 003–013
References
Apopa PL, Qian Y, Shao R. Iron oxide nanoparticles induce human microvascular endothelial cell permeability through reactive oxygen species production and microtubule remodeling Part Fibr Toxicol. [Internet] 2009; Jan 9 [cited 2019 Nov 18]; 6:1. Available from: https://particleandfibretoxicology.biomedcentral.com/articles/10.1186/1743-8977-6-1 CrossRef
Asahina E, Shimada K, Hisada J. A stable state of frozen protoplasm with invisible intracellular ice crystals obtained by rapid cooling. Exp Cell Res. 1970; 59(2): 349-58. CrossRef
Babiychuk VG, Marchenko VS, Babiychuk GA, et al. Structural and functional effect mechanisms of extreme cooling on hypothalamus thermoregulatory centers. Problems of Cryobiology. 2004; (2): 62-70. Full Text
Beck M, Baumeister W. Cryo-electron tomography: can it reveal the molecular sociology of cells in atomic detail? Trends Cell Biol. 2016; (26): 825-37. CrossRef
Drokin SI, Stain H, Govorukha TP. Ultrastructure of carp Cyprinus carpio spermatozoa after cooling, dilution and freeze-thawing. CryoLetters. 2002; (24): 49-55. PubMed
Farrant J, Walter CA, Heather L, McGann LE. Use of two-step cooling procedures to examine factors influencing cell survival following freezing and thawing. Cryobiology. 1977; 14(3): 273-86. CrossRef
Fleck RA. Low-temperature electron microscopy: techniques and protocols. Methods Mol Biol . 2015; 1257: 243-74. CrossRef
Fujikawa S. Freeze-fracture and etching on membrane damage on humane erythrocytes caused by formation of intracellular ice. Cryobiology. 1980; (12): 351-62. CrossRef
Goltsev AM. [Cryobiological pedestal of the Nobel Prize in chemistry for 2017]. Visn Nac Akad Nauk Ukr. 2018. (6): 75-85. Ukrainian. CrossRef
Gordienko EA, Pushkar. NS. [Physical foundations of low-temperature preservation of cell suspensions]. Kyiv: Naukova Dumka, 1994. 144 p. Russian
Gulevskyy АК, Repin NV, Schenyavsky II. Impairment of barrier properties of erythrocyte membranes caused by low temperatures is a result of disorganization of hemoglobin supramolecular structure. CryoLetters. 2016; 37(5): 357-64. PubMed
Hurbain I, Sachse M. The future is cold: cryo-preparation methods for transmission electron microscopy of cells. Biol Cell. 2011;103(9):405-20. CrossRef
Ignatyeva TA, Voyevodin VN, Goltsev AN, et al. Perspectives of constant gradient magnetic fields applications in biotechnology. Am J Biosci Bioeng. 2014; 2(6): 72-7. CrossRef
Kaprelyants AS, Marchenko LN, Matyash IP. [Ultrastructural analysis of liver destruction using freeze-substitution upon cryoapplication]. Kriobiologiya. 1986; (3): 36-9. Russian.
Kaprelyants OS, Marchenko LN, Migunova RK. Structure of liver sinusoid endothelial cells under the conditions of general cooling of rats. Problems of Cryobiology. 2003; (4): 70-6. Full Text
Kiroshka VV, Repin NV, Nadutov VM et al. [Synthesis, biological activity and cytotoxicity nanopowders based on Fe3O4]. Nanosystems, Nanomaterials, Nanotechnologies. 2010; 8(4): 787-98. Russian.
L'Azou B, Jorly J, Dinhill, et al. In vitro effects of nanoparticles on renal cells. Part Fibr Toxicol. [Internet] 2008; Dec 19 [cited 2019 Sep 21]; 5:22. Available from: http://www.particleandfi bretoxicology.com/content/5/1/22 CrossRef
MacKenzie AP, Luyet BJ. Electron microscope study of recrystallization in rapidly frozen gelatin gels. Biodynamica. 1967; 10(206): 95-122.
Marchenko VS, Babiychuk GA, Marchenko LN. [To fractal mechanisms of the structural and functional state of thermoregulation centers under hypothermia and hibernation]. Problems of Cryobiology. 2005; 15(3): 503-8. Ukrainian Full Text
Nei T. Growth of ice crystals in frozen specimens. J Micros. 1973; 22 (3): 227-33. CrossRef
Pavlovych OV, Hapon HO, Yurchuk TO, et al. Ultrastructural and functional characteristics of human spermatozoa after cryopreservation by vitrification. Probl Cryobiol Cryomed. 2020; 30 (1): 24-33. CrossRef
Pushkar NS, Kaprelyants AS, Pankov EYa. [Cell ultrastructure at low temperatures]. Kyiv: Naukova Dumka, 1978. 144 p. Russian.
Repin NV. [Study of extra- and intracellular crystallization in human erythrocytes under different cooling conditions]. Kriobiologiya. 1986. (3): 31-6. Russian.
Repin NV. To the question about two-step rapid freezing method. Estimation of aqueous membrane permeability in erythrocytes at temperature exposure stage. CryoLetters. 2009; (4): 251-61. PubMed
Repin NV, Bobrova EN, Repina SV. Temperature-induced transformation of mammalian red blood cells during hyperthermia. J Bioelectrochemistry. 2008; 73 (2): 101-5. CrossRef
Repin NV, Repina SV. [The ultrastructural and dynamic characteristics of erythrocyte membranes. The effect of the physiological status and temperature]. Tsitologiia. 1990. 32(11): 1094-8. Russian. PubMed
Repin NV, Skornyakov BA. [Methodological features and technical support of the method of freeze-fracturing]. Kriobiologiya i Kriomeditsina. 1982; (10): 89-92. Russian.
Repin NV, Yurchenko TN. [The role of environmental factors and the duration of exposure at 4°C in maintaining the shape of erythrocytes and their membranes. The mechanism of vesicle formation]. In: Goltsev AN, editor. [Actual problems of cryobiology and cryomedicine.] Kharkiv; 2012. p. 165-206. Russian.
Robards AW, Sleytr UB. Low temperature methods in biological electron microscopy (Practical methods in electron microscopy). Amsterdam-New York-Oxford: Elsevier, 1985. 551 p. 30. Staehelin A, Bertaud WS. Temperature and contamination dependent freeze-etch images of frozen water and glycerol solutions. J Ultrastr Research. 1971; 37 (1-2): 146-68. CrossRef
Suzdalev IP. [Nanotechnology: physical-chemistry of nanoclusters, nanostructures and nanomaterials]. Moscow: KomKniga, 2006. 592 p. Russian.
Watari F, Takashi N, Yokoyama A, et al. Material nanosizing effect on living organisms: non-specific, biointeractive, physical size effects. J R Soc Interface. 2009; 6: 371-88. CrossRef
Yurchenko TN, Kozlova VF, Skornyakov BA, et al. [Influence of cryoprotectants on biological systems]. Kyiv: Naukova Dumka, 1989. 240 p. Russian.
Zagnojko VI, Nardid OA, Lugovoj VI, Govorukha TP. Relationship between the degree of lysosome and mitochondria structural changes on freeze-thawing and the release of protein synthesis inhibitor. CryoLetters 1985; (6): 151-62.
Zinchenko AV, Mank VV, Ovcharenko FD, et al. [Structure and phase states of water-glycerol solutions]. Reports of the National Academy of Sciences of Ukraine. Ser B. 1982; (8): 38-42. Russian.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
