Osmotic Resistance of Rat’s Erythrocytes After Local Cold Injury
DOI:
https://doi.org/10.15407/cryo32.01.024Keywords:
local cold injury, erythrocytes, osmotic resistance, hypotonic shock, reactive termAbstract
The effect of local cold injury in rats on the osmotic resistance of erythrocytes in the early and late reactive terms was investigated. Cryoinfluence was performed by pressing the applicator to the skin on the lateral surface of the rat thigh for 30, 60 and 120 s. Afterwards, the blood was collected from the aorta of animals after 1 and 4 hours and after 24 and 48 hours (early and late reactive periods, respectively). The effect of hypotonic solutions on cells was used as a test for osmotic resistance of erythrocytes. It is shown that the osmotic resistance of erythrocytes in the early reactive term increases (compared with control cells). In the late reactive term, the indices of osmotic resistance of erythrocytes after 24 hours approached the control values and after 48 hours they reached the norm. Osmotic resistance of erythrocytes in both reactive terms after local cold injury does not depend on the duration of cryoinfluence (30, 60, 120 s). The findings are considered from the standpoint of adaptive reactions that have an allostatic nature.
Probl Cryobiol Cryomed 2022; 32(1): 024–033
References
Andrews MD. Cryosurgery for common skin conditions. Am Fam Physician. 2004; 69 (10): 2365-72.
Arias M, Quijano JS, Haridas V, et al. Red blood cell permeabilization by hypotonic treatments, saponin, and anticancer avicins. Biochim Biophys Acta. 2010; 1798 (6): 1189-96. CrossRef
Baust JG, Gage AA, Bjerklund JTE, Baust JM. Mechanisms of cryoablation: clinical consequences on malignant tumors. Cryobiology. 2014; 68 (1): 1-11. CrossRef
Bolton-Maggs PHB, Langer JC, Iolascon A, et al. Guidelines for the diagnosis and management of hereditary spherocytosis - 2011 update. Br J Haematol. 2011; 156: 37-49. CrossRef
De Freitas MAR, da Costa AV, Medeiros LA, et al. The role of the erythrocyte in the outcome of pregnancy with preeclampsia. PLoS One [Internet]. 2019 Mar 6 [cite 13.07.2021]; 14 (3): e0212763. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212763 CrossRef
De Freitas MV, Marquez-Bernardes L F, de Arvelos LR, et al. Influence of age on the correlations of hematological and biochemical variables with the stability of erythrocyte membrane in relation to sodium dodecyl sulfate. Hematology. 2014; 19(7): 424-30. CrossRef
Eder PS, Soong CJ, Tao M. Phosphorylation reduces the affinity of protein 4.1 for spectrin. Biochemistry. 1986; 25 (7): 1764-70. CrossRef
Garmaeva DK, Arzhakova LI, Dmitrieva TI, et al. [Indicators of cellular composition at experimental cold effects.] Modern problems of science and education. 2019; (1): 1-8. Russian.
Gordienko EA, Gordienko YuE, Gordienko OI. The physico-mathematical theory of human erythrocyte hypotonic hemolysis phenomenon. CryoLetters. 2003; 24 (4): 229-44. PubMed
Ishikawa Y, Eguchi T, Skowronski MT, Ishida H. Acetylcholine acts on M3 muscarinic receptors and induces the translocation of aquaporin 5 water channel via cytosolic Ca2+ elevation in rat parotid glands. Biochem Biophys Res Commun. 1998; 245 (3): 35-40. CrossRef
Krysova AV, Nozdrachev AD, Kunshin AA, Tsirkin VI. [The effect of alpha- and beta- adrenoblockers on the ability of adrenaline to change the osmotic resistance of erythrocytes of nonpregnant women]. Vestnik of Saint Petersburg University. Series 3. Biology. 2013; (1): 54-68. Russian.
Kuchuk EА. [From stress to resilience.] Zhurnal nevrolohii im. B.M. Mankovskoho. 2016; 4 (1): 72-6. Russian.
Lew VL, Daw N, Etzion Z, et al. Effects of age-dependent membrane transport changes on the homeostasis of senescent human red blood cells. Blood. 2007; 110 (4): 1334-42. CrossRef
Mascarenhas Netto R de C, Fabbri C, de Freitas MV, et al. Influence of Plasmodium vivax malaria on the relations between the osmotic stability of human erythrocyte membrane and hematological and biochemical variables. Parasitol Res. 2014; 113(3): 863-74. CrossRef
McEwen BS. Central effects of stress hormones in health and disease: Understanding the protective and damaging effects of stress and stress mediators. Eur J of Pharmacol. 2008; 583 (2-3):174-85. CrossRef
McEwen BS, Wingfield JC. What is in a name? Integrating homeostasis, allostasis and stress. Horm Behav. 2010; 57 (2): 105-11. CrossRef
Muravyov AV, Tikhomirova IA, Akhapkina AA, et al. [Micromechanical responses of human red blood cells to stimulation of membrane receptors, ionic channels and enzymes.] Rossiyskiy zhurnal biomekhaniki. 2016; 20 (1): 23-30. Russian.
Oliynyk GA. [Pathophysiology of cold shock.] Medytsyna nevidkladnykh staniv. 2013; (3): 16 - 21. Ukrainian. CrossRef
Panin LE, Mokrushnikov PV, Kunitsyn VG, et al. [Fundamentals of multilevel mesomechanics of nanostructural transitions in erythrocyte membranes and their destructions in interaction with stress hormones.] Fizicheskaya mezomekhanika. 2011; 14 (3 - 4): 167-77. Russian. CrossRef
Penha-Silva N, Firmino CB, de Freitas Reis FG, et al. Influence of age on the stability of human erythrocyte membranes. Mech Ageing Dev. 2007; 128 (7-8): 444-9. CrossRef
Pretini V, Koenen MH, Kaestner L, et al. Red blood cells: Chasing interactions. Front Physiol [Internet]. 2019 Jul 31 [cited 21.07.2021]. 10: 945. Published 2019 Available from: https://www.frontiersin.org/articles/10.3389/fphys.2019.00945/full CrossRef
Rodrigues R, de Medeiros LA, Cunha LM, et al. Correlations of the glycemic variability with oxidative stress and erythrocytes membrane stability in patients with type 1 diabetes under intensive treatment. Diabetes Res Clin Pract. 2018; 144: 153-160. CrossRef
Semionova EA, Iershova NA, Orlova NV, Shpakova NM. Hypotonic lysis of mammalian erythrocytes in chlorpromazine presence. Eastern European Scientific Journal. 2016; (2): 7-17.
Yasui H, Kubota M, Iguchi K, et al. Membrane trafficking of aquaporin 3 induced by epinephrine. Biochem Biophys Res Commun. 2008; 373 (4): 613-7. CrossRef
Walski T, Chludzinska L, Komorowska M, Witkiewicz W. Individual osmotic fragility distribution: a new parameter for determination of the osmotic properties of human red blood cells. Biomed Res Int [Internet]. 2014 Jan 2 [cite 21.07.2021]. 2014: 162102. Available from: https://www.hindawi.com/journals/bmri/2014/162102 CrossRef
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