Lyophilized Human Cord Blood Leukoconcentrate to Treat Brain Ischemia in Rats

Authors

  • Volodymyr V. Lebedinets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv
  • Ludmila V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Mykola O. Bondarovych Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7977-0283
  • Olena D. Lutsenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center for Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Academy of Medical Sciences and Ministry of Health Care of Ukraine, Kharkiv https://orcid.org/0000-0003-3982-9687
  • Dmytro V. Lebedinets V.N. Karazin Kharkiv National University, Kharkiv
  • Igor G. Grisha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-2875-910X
  • Maksym V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-8412-5262
  • Kyrylo Yu. Parkhomenko Municipal Health Care Institution ‘Regional Clinical Hospital – Center for Emergency Care and Disaster Medicine’, Kharkiv
  • Anatoliy M. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; Interdepartmental Scientific Center for Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Academy of Medical Sciences and Ministry of Health Care of Ukraine, Kharkiv https://orcid.org/0000-0002-5289-5876

DOI:

https://doi.org/10.15407/cryo32.01.044

Keywords:

lyophilization, cryopreservation, human cord blood leukoconcentrate, ischemic stroke, immune system, interferon-γ, interleukin-10, adhesive activity, phagocytic activity

Abstract

The paper outlines the experimental data on the impact of lyophilization on preservation of structural and functional characteristics of cells from human cord blood leukoconcentrate (HCBL). Lyophilization of HCBL was shown to ensure the preservation of cells with immune modulating potential in a heterogeneous population of HCBL. Lyophilized HCBL (lHCBL), to the same extent as cryopreserved one (cHCBL), revealed an equal immune correcting effect during ischemic brain injury in vivo in the experimental model of ischemic stroke (IS). The inclusion of lHCBL in therapy of brain ischemia demonstrated the improved indices of IFN-ү+ and IL-10+ spleen cells, adhesive and phagocytic activity of peritoneal cavity cells in rats with IS. The efficiency of using both lHCBL and cHCBL during IS may be associated with the implementation of therapeutic effect by presented among them cells and mediators, including multi-vector regulatory systems that maintain homeostatic stability of the body (immune, endocrine, nervous ones).

 

Probl Cryobiol Cryomed 2022; 32(1): 044–057

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Published

2022-06-03

How to Cite

Lebedinets , V., Ostankova , L., Bondarovych , M., Lutsenko , O., Lebedinets , D., Grisha , I., Ostankov, M., Parkhomenko , K., & Goltsev, A. (2022). Lyophilized Human Cord Blood Leukoconcentrate to Treat Brain Ischemia in Rats . Problems of Cryobiology and Cryomedicine, 32(1), 44–57. https://doi.org/10.15407/cryo32.01.044

Issue

Vol. 32 No. 1 (2022): Probl Cryobiol Cryomed

Section

Cryomedicine, Clinical and Experimental Transplantology

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