Correction of Cytokine Profile in Autoimmune Diseases with Embryofetoplacental Complex Cryopreserved Products

Authors

  • Anatoliy M. Goltsev 1. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; 2. Interdepartmental Scientific Center for Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Academy of Medical Sciences and Ministry of Health Care of Ukraine, Kharkiv https://orcid.org/0000-0002-5289-5876
  • Olena D. Lutsenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-3982-9687
  • Kateryna Ye. Yampolska Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Yuliia O. Gaevska Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-2375-357X
  • Mykola O. Bondarovych Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7977-0283
  • Lyudmyla V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Larisa V. Sokil Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-8702-7374
  • Lyudmyla V. Stepaniuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-6491-8791
  • Ihor G. Grisha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0003-2875-910X

Keywords:

adjuvant arthritis, cytokines, lyophilization, human cord blood leukoconcentrate, placental cryoextract

Abstract

In this work, we experimentally substantiated the possibility of correcting the cytokine profile to reduce the inflammation in mice with adjuvant arthritis (AA) via combined use of lyophilized human cord blood leukoconcentrate (lHCBL) and cryopreserved human placental extract (cHPE). Adjuvant arthritis was modelled in CBA/H mice. To day 7 after pathology induction, the animals were injected with lHCBL and cHPE singly or in combination. To days 14 and 28 of observation, all the introduced products showed a therapeutic effect. To 28 day of observation, the efficiency of combined use of lHCBL and cHPE was 3.1 and 2.5 times higher vs. the monotherapy (respectively), likely resulting from their synergy in respect to a decrease in arthritis index, TNFα concentration, impact of lHCBL and cHPE on IFNγ and IL-6 levels, correspondingly. 

Probl Cryobiol Cryomed 2022; 32(2):121133

Author Biographies

Anatoliy M. Goltsev, 1. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; 2. Interdepartmental Scientific Center for Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Academy of Medical Sciences and Ministry of Health Care of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Olena D. Lutsenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Kateryna Ye. Yampolska, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Yuliia O. Gaevska , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Mykola O. Bondarovych , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Lyudmyla V. Ostankova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Larisa V. Sokil , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Lyudmyla V. Stepaniuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

Ihor G. Grisha , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of cryopathophysiology and immunology

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Published

2022-10-30

How to Cite

Goltsev, A., Lutsenko, O., Yampolska, K., Gaevska , Y., Bondarovych , M., Ostankova, L., Sokil , L., Stepaniuk , L., & Grisha , I. . (2022). Correction of Cytokine Profile in Autoimmune Diseases with Embryofetoplacental Complex Cryopreserved Products . Problems of Cryobiology and Cryomedicine, 32(2), 121–133. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1793

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Section

Cryomedicine, Clinical and Experimental Transplantology