Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of its Extracts

Authors

  • Olena M. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-9100-1823
  • Yevheniia Yo. Naumenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-9727-3165
  • Myroslav I. Shchetinskyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-9279-2695
  • Stanislav V. Narozhnyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-9690-0203
  • Оleg А. Nardid 1 Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; 2 V. N. Karazin Kharkiv National University, Kharkiv https://orcid.org/0000-0002-9439-0606
  • Мarina М. Kalashnykova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7813-7208
  • Irina I. Shchetinska National Technical University ‘Kharkiv Polytechnic Institute’, Kharkiv

Keywords:

low-temperature preservation, human placenta, aqueous-saline extracts, erythrocyte thermohemolysis, platelet aggregation, burns, wound healing effect

Abstract

Here we have studied the effect of low-temperature storage of the placenta at different temperatures on anti-inflammatory activity of its extracts in cell models of erythrocyte thermohemolysis and adenosine-5’-diphosphate-induced platelet aggregation. The wound-healing effect of cryopreserved placental extracts was also evaluated in rat thermal burn model. When preserving placenta for up to a month, the temperature of storage was shown to cause no significant impact on the percentage of inhibition of erythrocyte thermohemolysis and platelet aggregation by the extracts. Placental storage for three months at –20°C decreased anti-inflammatory activity of its extracts, and led to its complete loss during 6 months. Placental cryopreservation at –196°C enabled to preserve the anti-inflammatory and wound-healing activity. The cryopreserved placenta-derived extracts showed a pronounced positive effect on reparative process at third-degree (B) skin burn injury in rats.

Probl Cryobiol Cryomed 2022; 32(2):144157

Author Biographies

Olena M. Bobrova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Yevheniia Yo. Naumenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Myroslav I. Shchetinskyi , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Stanislav V. Narozhnyi , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Оleg А. Nardid , 1 Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; 2 V. N. Karazin Kharkiv National University, Kharkiv

Department of Cryobiophysics

Мarina М. Kalashnykova , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

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Published

2022-10-30

How to Cite

Bobrova, O., Naumenko , Y., Shchetinskyi , M., Narozhnyi , S., Nardid О. ., Kalashnykova М., & Shchetinska , I. . (2022). Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of its Extracts . Problems of Cryobiology and Cryomedicine, 32(2), 144–157. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1795

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Section

Cryomedicine, Clinical and Experimental Transplantology