Сryopreservation Effect on Proliferation Characteristics and Differentiation Potential of Cultured Chorion Cells

Authors

  • E. I. Goncharuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Volkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, chorion cell culture, viability, proliferation, differentiation

Abstract

Five programs of chorion cell culture (ChCC) cryopreservation which differ by the cooling rate and duration have been studied in the work. The properties such as viability, adhesion, proliferation and directed differentiation were studied using culturing, light and fluorescence microscopy. The program comprising the cooling down to –6°C with the rate of 1°C/min, seeding with following cooling down to –80°C with the rate of 10°C/min provides both high integrity of ChCC population and survival of the fraction of multipotent cells.

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Published

2010-09-20

How to Cite

Goncharuk, E. I., Volkova, N. A., & Grischenko, V. I. (2010). Сryopreservation Effect on Proliferation Characteristics and Differentiation Potential of Cultured Chorion Cells. Problems of Cryobiology and Cryomedicine, 20(3), 309–317. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/191

Issue

Vol. 20 No. 3 (2010): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 E. I. Goncharuk, N. A. Volkova, V. I. Grischenko

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