Temperature Dependence of Testes Intersticium Cell Membrane Permeability for Cryoprotectant Molecules

Authors

  • N. A. Chernobai Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. Ye. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

permeability coefficients, activation energy, testis intersticium cells, volumetry, cryoprotectants

Abstract

Osmotic behavior of the rat testis intersticium cells in cryoprotectants solutions – substances belonging to alcohols (ethylene glycol (EG), glycerol and 1,2-butane diol (1,2-BD)), oxides (dimethyl sulfoxide (Me2SO)) and amides (dimethyl formamide (DMFA)) was studied. Permeability coefficients of testis cells for these cryoprotectants were determined at 35, 20 and 5°C by volumetric analysis and the Kedem-Kachalsky modified physico-mathematical model. The calculated values of the activation energies for EG, glycerol, 1,2-BD, Me2SO and DMFA molecules are 53.28, 46.92, 36.33, 58.89, 62.99 kJ/mol, respectively. Testis cell membranes were established to be equally well permeable for EG, glycerol, 1,2-BD and Me2SO, and their permeability for DMFA at 35°C was comparable with that for water molecules.

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Published

2010-06-07

How to Cite

Chernobai, N. A., Pakhomov, A. V., Kovalenko, I. F., Bozhok, G. A., Kovalenko, S. Y., & Rozanov, L. F. (2010). Temperature Dependence of Testes Intersticium Cell Membrane Permeability for Cryoprotectant Molecules. Problems of Cryobiology and Cryomedicine, 20(2), 153–158. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/204

Issue

Vol. 20 No. 2 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. A. Chernobai, A. V. Pakhomov, I. F. Kovalenko, G. A. Bozhok, S. Ye. Kovalenko, L. F. Rozanov

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