Platelet Cryopreservation. 1. Development of Cryopreservative Solutions, Based on Cryoprotectant Combination and Study of Their Efficiency

Authors

  • O. A. Bogdanchikova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

platelets, cryopreservation, combined cryoprotective solutions, aggregation, hypotonic shock reaction, platelet clot retraction

Abstract

Cryoprotective efficiency of cryopreservative solutions, based on combining different cryoprotectants during platelet freezing, was studied in the research. There were established the cryoprotectant combinations such as: 10% dimethyl acetamide (DMAC)/1,2-propane diol (1,2-PD), DMAC/glycerol and DMAC/oxyethylated glycerol (n = 5), significantly increasing the integrity of cryopreserved platelets, compared to the results of freezing under protection of monocryoprotective media, containing one of the mentioned above substances, including 10% dimethyl sulfoxide.

Downloads

Published

2009-11-30

How to Cite

Bogdanchikova, O. A., & Kompaniets, A. M. (2009). Platelet Cryopreservation. 1. Development of Cryopreservative Solutions, Based on Cryoprotectant Combination and Study of Their Efficiency. Problems of Cryobiology and Cryomedicine, 19(3), 324–337. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/256

Issue

Vol. 19 No. 3 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 O. A. Bogdanchikova, A. M. Kompaniets

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).