Effect of Human Cord Blood Leukoconcentrate on Skin Regenerative Activity in Culture In Vitro

Authors

  • A. A. Tsutsayeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Volina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. V. Sokol Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. A. Zhulikov Kharkov Municipal Clinical Hospital N31, Kharkov

Keywords:

skin explants, human cord blood leukoconcentrate, in vitro culturing, morphofunctional activity

Abstract

The effect of human cord blood cryopreserved cell leukoconcentrate, suspended in autologous plasm (LHCB preparation) on in vitro cultured human skin morphology in different terms, has been studied. Skin was cultured on an agarised medium, supplemented with 17% embryonic serum (control) and 10% LHCB preparation (experimental group). The skin viability in vitro was maintained for 5–7 days longer in the presence of LHCB preparation compared to the control group. Under LHCB effect in cultured skin flaps there was an increase in fibroblast and fibrous structure amount, that enables concluding about the stimulation of both processes of fibroblast proliferation and synthesis of fibrous structures in derma.

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Published

2009-05-25

How to Cite

Tsutsayeva, A. A., Volina, V. V., Sokol, L. V., & Zhulikov, O. A. (2009). Effect of Human Cord Blood Leukoconcentrate on Skin Regenerative Activity in Culture In Vitro. Problems of Cryobiology and Cryomedicine, 19(1), 93–99. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/291

Issue

Vol. 19 No. 1 (2009): Problems of Cryobiology

Section

Cryomedicine, Clinical and Experimental Transplantology

License

Copyright (c) 2020 A. A. Tsutsayeva, V. V. Volina, L. V. Sokol, O. A. Zhulikov

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