Technological Aspects for Cryopreservation of Enzyme-Based Biosensors

Authors

  • Oleg A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Dmitriy O. Mangasarov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine
  • Miroslav I. Schetinskiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Keywords:

long-term storage, enzyme biosensor, device for freezing, cassette holder, low temperature containers

Abstract

Basing on the proposed approaches for storage of enzyme-based biosensors a technological process was developed, which allowed rapid and easy freezing, storage and warming of these devices. To do this we designed and produced the samples of containers, holder-cassettes, equipment for freezing according to the needed regimens. The produced equipment was involved to creation of technology for long-term storage of miniature glucose oxidase-based electrochemical biosensors. Probl Cryobiol Cryomed 2013; 23(4):338–346.

Author Biographies

Oleg A. Nardid, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiophysics

Dmitriy O. Mangasarov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiophysics

Miroslav I. Schetinskiy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov, Ukraine

Department of Cryobiophysics

References

Biosensors: bases and applications/ Ed. by E. Turner, I. Karube, J. Wilson. Moscow: Mir, 1992.

Grischenko V.I., Nardid O.A., Rozanova K.D. et al. Low temperature stabilization of glucose oxidase as component of biological sensor. Biopolymery I Klityna 2006; 22 (3): 236–242.

Dzyadevich S.V., Soldatkin O.P. Scientific and technological grounds of creating miniature electrochemical biosensors. Kyiv: Naukova Dumka, 2006.

Mosolov V.V., Sokolova E.V. Interaction of glycols and glycerol with active center of trypsin and chymotrypsin. Doklady AN SSSR 1972; 207(1): 91–93.

Naumenko E.I., Rozanova E.D. Study of mechanisms of cryodamage of isolated cytochrome oxidase. In: The Second All-Union Conference on Theoretical and Applied Questions of Cryobiology, October 9–11, 1984: Proceeding. Kharkov, 1984: 61.

Rozanova E.D., Moiseyev V.A., Naumenko E.I. Effect of freeze–thawing on structure and function of cytochrome oxidase. Ukr Biokhim Zhurnal 1985; 57 (1): 61–64.

Soldatkin O.P. Development of scientific and technological bases for creation of electrochemical biosensors for demands of medicine, biotechnology and environmental protection [Author’s abstract … Doct. Biol. Sci.]. Kiev, 1998.

Yurchenko T.N., Kozlova V.F., Skornyakov B.A. Effect of cryoprotectants on biological systems. Kiev: Naukova Dumka, 1989.

Patent of Ukraine N23507 IPC F25B19/00. Device for freezing biomaterials/ D.O. Mangasarov, O.A. Nardid, K.D. Rozanova et al. Appl. 22.01.2007; Publ. 25.05.2007, Bul. N7.

Patent of Ukraine N70805A IPC A01N1/12. Container for low temperature preservation of biological objects/ V.I. Grischenko, O.T. Khodko, O.S. Prokopyuk et al. App. 29.12. 2003; Publ. 15.10.2004, Bul. 10.

Climent P.V., Serralheiro M.L.M., Rebelo M.I.F. Development of a new amperometric biosensor based on polyphenoloxidase and polyethersulphone membrane. Pure Appl Chem 2001; 73 (12): 1993–1999.

Korpan Y.I., Dzyadevich S.V., Zharova V.P., El'skaya A.V. Conductometric biosensor for ethanol detection based on whole yeast cells. Ukr Biokhim Zhurnal 1994; 66 (1): 78–82.

Market C. L. Lactate degidrogenaze izozymes: Dissociation and recombination of subunits. Science 1963; 140 (3573): 341–350.

Pancrasio J.J.,WhelanJ.P., Borkholder D.A. et al. Development and application of cell-based biosensors. Ann Biomed Eng 1999; 27: 697–711.

Sanders C.A., Rodriguez M.Jr., Greenbaum E. Stand–off tissue based biosensors for the detection of chemical warfare agents using photosynthetic fluorescence induction. Biosens Bioelectron 2001; 16: 439–446.

Santos A.S., Freire R.S., Kubota L.T. Highly stable amperometric biosensor for ethanol based on Meldola's blue adsorbed on silica gel modified with niobium oxide. J Electroanal Chem 2003. – 547 (2): 135–142.

Whittman J.H., Rosano H.L. Effects of freeze-thaw process on amylase. Cryobiology 1973; 10 (3): 240–243.

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Published

2013-12-30

How to Cite

Nardid, O. A., Mangasarov, D. O., & Schetinskiy, M. I. (2013). Technological Aspects for Cryopreservation of Enzyme-Based Biosensors. Problems of Cryobiology and Cryomedicine, 23(4), 338–346. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/319