Effect of Microinjection of Cryoprotective Agents on Survival of Misgurnus Fossilis L. Loach Embryos, 1758

Authors

  • K. B. Mikson Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Ye. F. Kopeyka Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. I. Grischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

embryo, loach (Misgurnus fossilis L.), microinjection, cryoprotective agents

Abstract

Investigated survival rate of loach embryos at a stage late epibole after a microinjection of DMSO, PEG-100, PEG-1500 and formamide (FA) cryoprotectants in area of perivitelline spaces (PS) and yolk sac (YS). It is established that at introduction of DMSO, PEG-100 and PEG-1500 in concentration of 10, 50 and 95 % in area of PS and at the subsequent 30-minute incubation in 0,88 and 0,29 M to sucrose, survival rate level decreased: 58,4–48,5, 74,4–53,9 and 79,63–59,6 % accordingly. At FA survival rate level under the same conditions decreased from 7 to 3,6 %. At introduction of PEG-100 and PEG-1500 in concentration 10, 50 and 95 % in YS area at 30-minute incubation in 0,88 and 0,29 M to sucrose level of survival rate had no statistically significant differences and was higher, than at introduction of DMSO and FA.

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Published

2008-12-22

How to Cite

Mikson, K. B., Kopeyka, Y. F., & Grischenko, V. I. (2008). Effect of Microinjection of Cryoprotective Agents on Survival of Misgurnus Fossilis L. Loach Embryos, 1758. Problems of Cryobiology and Cryomedicine, 18(4), 509–514. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/365

Issue

Vol. 18 No. 4 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 K. B. Mikson, Ye. F. Kopeyka, V. I. Grischenko

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