Character of Changes of Structural and Functional Parameters of Fetal Liver Cells after Cryopreservation Depending on Their Gestation Terms

Authors

  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. V. Rassokha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fetal liver cells, hemopoietic stem cells, C3H mice, breast cancer

Abstract

The efficiencies of three freezing programs (two, three- and four-stage) basing on the estimation of morphofunctional state and therapeutic potential of cryopreserved fetal liver cells (СFLCs) of 15 and 19 gestation days were studied. The investigations have shown that during freezing of FLC-15 the most effective was two-stage cryopreservation regimen under 10% DMSO protection, which provided higher content of survived and hemopoietic stem cells (CD34+CD38–). From this point of view the most suitable for cFLC-19 was the use of 12.5% DMSO at three-stage freezing program. In C3H mice genetically determined to the development of breast cancer the most manifested reduction of the frequency of tumor development and higher survival were observed after application of cFLC-15.

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Published

2008-11-20

How to Cite

Ostankov, M. V., Bondarovich, N. A., Rassokha, I. V., & Goltsev, A. N. (2008). Character of Changes of Structural and Functional Parameters of Fetal Liver Cells after Cryopreservation Depending on Their Gestation Terms. Problems of Cryobiology and Cryomedicine, 18(3), 302–305. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/395

Issue

Vol. 18 No. 3 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 M. V. Ostankov, N. A. Bondarovich, I. V. Rassokha, A. N. Goltsev

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