Effect of Sugars on Dehydration of Biopolymer Molecules During Cryopreservation

Authors

  • A. A. Rugal Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • V. N. Barvinchenko Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • N. P. Galagan Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • I. V. Siora Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • V. V. Turov Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev

Keywords:

cryopreservation, cryoprotectants, NMR-spectroscopy, hydration ability, bound water, interfacial energy

Abstract

Protein molecules’ hydration in the presence of sugars: glucose, fructose and sucrose were studied by NMR-spectroscopy. The parameters of hydration, concentration of bound water and value of interfacial energy were determined over a wide range of component concentrations. It was shown with exemplifying using the serum albumin, that low-molecular compounds (sugars) could replace an intraglobular water. The maximal effect of dehydration was observed for sucrose. In this case protein molecules change their property and capability to be adsorbed on a surface of fine-grained silica, which is reduced by 25% as a result of the forming complexes.

Downloads

Published

2007-12-15

How to Cite

Rugal, A. A., Barvinchenko, V. N., Galagan, N. P., Siora, I. V., & Turov, V. V. (2007). Effect of Sugars on Dehydration of Biopolymer Molecules During Cryopreservation. Problems of Cryobiology and Cryomedicine, 17(4), 374–384. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/484

Issue

Vol. 17 No. 4 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. A. Rugal, V. N. Barvinchenko, N. P. Galagan, I. V. Siora, V. V. Turov

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).