Analysis of Effect of Different Steps of Vitrification Protocol for Cryopreservation in Ethylene Glycol-Sucrose Medium on 2-Cell Murine Embryo Viability

Authors

  • Ye. I. Smolyaninova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. V. Pishko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. G. Lisina Institute of Cattle Breeding of Ukrainian Academy of Agrarian Sciences, Kharkov
  • A. A. Kolesnikova Institute of Cattle Breeding of Ukrainian Academy of Agrarian Sciences, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

vitrification, 2-cell murine embryo, ethylene glycol, sucrose, cryopreservation

Abstract

The effect of different stages of vitrification protocol for cryopreservation with ethylene glycol-sucrose medium on morphological integrity and viability of 2-cell murine embryos has been studied. This procedure was shown as enabling to preserve viable more than 70% embryos. The stages of exposure in freezing medium and freeze-thawing are mostly responsible for embryo cryoinjury. Basing on the literature reports and own data the role of osmotic and mechanical factors in cell injury at the rapid freezing stages is discussed.

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Published

2007-12-15

How to Cite

Smolyaninova, Y. I., Pishko, O. V., Lisina, E. G., Kolesnikova, A. A., & Rozanov, L. F. (2007). Analysis of Effect of Different Steps of Vitrification Protocol for Cryopreservation in Ethylene Glycol-Sucrose Medium on 2-Cell Murine Embryo Viability. Problems of Cryobiology and Cryomedicine, 17(4), 385–393. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/485

Issue

Vol. 17 No. 4 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 Ye. I. Smolyaninova, O. V. Pishko, E. G. Lisina, A. A. Kolesnikova, L. F. Rozanov

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