Effect of Rapid Two-Step Freezing on Bone Marrow Cell Integrity

Authors

  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

rapid two-step freezing, supercooling, intracellular crystallisation, dimethyl sulfoxide

Abstract

Supercooling of murine bone marrow intracellular solution under cell suspension cooling with a constant rate and at a rapid twostep
freezing has been theoretically calculated. The optimal conditions for these cells cryopreservation under dimethyl sulfoxide protection have been determined, basing on the calculations and experiments performed.

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Published

2007-10-25

How to Cite

Ostankov, M. V. (2007). Effect of Rapid Two-Step Freezing on Bone Marrow Cell Integrity. Problems of Cryobiology and Cryomedicine, 17(3), 283–289. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/503

Issue

Vol. 17 No. 3 (2007): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 M. V. Ostankov

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