PEO-1500 Inhibits Human Erythrocyte Ca2+-ATPase

Authors

  • N. G. Zemlyanskikh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Nikolchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocytes, Ca2 -ATPase, cryopreservation, cryoprotectant, polyethylene oxide

Abstract

Human erythrocyte Ca2+-ATPase provides the maintenance and regulation of intercellular calcium level. Change in its functional activity under cryopreservation plays an important role in cell survival and structural integrity preservation. PEO-1500 exocellular cryoprotectant inhibits erythrocyte Ca2+-ATPase activity at incubation stage but the following freeze-thawing cycle (–196...37°C) in its presence does not affect the settled regimen of this enzyme functioning. The inhibition caused by PEO-1500 effect has a reversible character and the erythrocyte Ca2+-ATPase activity is recovered after its dilution in cell suspension that is essential for cryopreserved cell normal functioning in blood bed after transfusion. The inhibition of Ca2+-ATPase activity is apparently associated to physical and chemical membrane modification under this compound effect.

Downloads

Published

2007-06-15

How to Cite

Zemlyanskikh, N. G., Nikolchenko, A. Y., & Babijchuk, L. A. (2007). PEO-1500 Inhibits Human Erythrocyte Ca2+-ATPase. Problems of Cryobiology and Cryomedicine, 17(2), 115–125. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/511

Issue

Vol. 17 No. 2 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. G. Zemlyanskikh, A. Yu. Nikolchenko, L. A. Babijchuk

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).