Study of the Effect of Cryoprotectants on Liposomes of Canine Spermatozoa Total Lipids

Authors

  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. I. Yegorov Kiev National University named by Taras Shevchenko
  • V. G. Pivovarenko Kiev National University named by Taras Shevchenko

Keywords:

dog, spermatozoa, lipids, cryoprotectants, fluorescent probe, FME

Abstract

The mechanism of interaction between the cryoprotective agents (CPAs): ethylene glycol (EG), glycerol and N,N-dimethylformamide (DMFA) with lipid bilayer of liposomes formed from total lipids of canine spermatozoa by fluorescent spectroscopy using new fluorescent probe of flavonoid origin: 3-hydroxy-4’-(N,N-dimethylamine)flavon (FME). It has been established that among the studied CPAs (glycerol, EG, DMFA) the highest effect on the form of FME fluorescence spectrum associated to lipid bilayer is rendered by DMFA. The effect rate of the studies CPAs on liposomes is determined by hydrophobic-hydrophilic balance of their molecules. DMFA predominantly affects lipid bilayer by hydrophobic interaction, and glycerol and EG mainly influence hydrate layer of polar “heads†of phospholipids by forming intermolecular hydrogen bonds.

Downloads

Published

2007-06-15

How to Cite

Linnik, T. P., Dyubko, T. S., Yegorov, M. I., & Pivovarenko, V. G. (2007). Study of the Effect of Cryoprotectants on Liposomes of Canine Spermatozoa Total Lipids. Problems of Cryobiology and Cryomedicine, 17(2), 141–146. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/513

Issue

Vol. 17 No. 2 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. P. Linnik, T. S. Dyubko, M. I. Yegorov, V. G. Pivovarenko

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).