Cryoresistance of Organotypic Cultures Derived from Adrenal Tissues of Rats of Various Ages

Authors

  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. Kh. Rumiekh V.N. Karazin Kharkov National University, Kharkov

Keywords:

cryopreservation, adrenal organotypic cultures, hormone secretion, viability, animal’s age

Abstract

The paper covers the studies in cryosensitivity of organotypic cultures derived from the adrenal tissues of white rats of various ages (1, 3, 6, 12 and 24 months ) based on the analysis of viable cells in culture prior to and after cryopreservation in 5% dimethyl sulfoxide presence. It has been established that secreting ability of culture cells depends on an age of the animal that the tissues were derived from. The cultures of tissues of aged animals (12-24 months) have lower activity and higher sensitivity to low temperature effect.

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Published

2007-06-15

How to Cite

Bondarenko, T. P., & Rumiekh, Y. K. (2007). Cryoresistance of Organotypic Cultures Derived from Adrenal Tissues of Rats of Various Ages. Problems of Cryobiology and Cryomedicine, 17(1), 44–49. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/530

Issue

Vol. 17 No. 1 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 T. P. Bondarenko, Yu. Kh. Rumiekh

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