Effect of Erythrocytes Incubation in Hypertonic Non-Electrolyte Media on their Osmotic Resistance

Authors

  • E. E. Nipot Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Melikhova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. A. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocytes, hypertonic shock, post-hypertonic lysis, valinomycine, non-electrolyte media

Abstract

Partial K+ loss by cell as a result of hypertonic incubation into 0.8-1.4 M sucrose solution has been established as contributing to an increase in erythrocyte osmotic resistance to hypotonic shock (50 mM NaCl solution). Cell sensitivity to a change in medium osmotic parameters after dehydration in valinomycine-containing non-electrolyte solutions (1mM final concentration) depends on a temperature of hypertonic incubation.

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Published

2006-12-15

How to Cite

Nipot, E. E., Melikhova, S. V., & Bondarenko, V. A. (2006). Effect of Erythrocytes Incubation in Hypertonic Non-Electrolyte Media on their Osmotic Resistance. Problems of Cryobiology and Cryomedicine, 16(4), 363–369. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/570

Issue

Vol. 16 No. 4 (2006): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 E. E. Nipot, S. V. Melikhova, V. A. Bondarenko

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