Animal Sperm Freezing Under Laboratory and Field Conditions

Authors

  • L. V. Gorbunov Institute of Animal Breeding of Ukrainian Academy of Agrarian Sciences, village Kulinichi, Kharkov region
  • A. S. Salina Institute of Animal Breeding of Ukrainian Academy of Agrarian Sciences, village Kulinichi, Kharkov region

Keywords:

cryopreservation of biological object, slow rates of heat exchange, fish sperm, device

Abstract

The device for cryopreservation of sexual cells of mammalians and fish under field conditions has been proposed. It provides freezing rates from 0.5 to 30ºC/min. The change in freezing rate is achieved with dosed intensity of heat-conducting path by means of “mantel†from metal container with biological object in X-35 Dewar vessel neck. Location of containers with biological objects in precooled thermal block helps to realize various behaviors of rate changes. Using the methods of mathematical modeling there have been chosen the materials and parameters of metal container providing minimum gradient of temperature distribution (≤1ºC/cm) during cooling that provides freezing of cell suspensions up to 5 ml in a container. The height location of “mantel†regarding metal container and depth of its submerging into X-35 Dewar vessel neck, necessary to realize optimal freezing regimens of bovine and fresh-water fish sperm have been established. Survival of frozen-thawed carp sperm made 45 and 40% for bovine ones.

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Published

2006-06-15

How to Cite

Gorbunov, L. V., & Salina, A. S. (2006). Animal Sperm Freezing Under Laboratory and Field Conditions. Problems of Cryobiology and Cryomedicine, 16(2), 147–154. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/595

Issue

Vol. 16 No. 2 (2006): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 L. V. Gorbunov, A. S. Salina

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