Morphological and Functional Assessment of Human Fetal Liver Cells in Long-Term Culture

Authors

  • P. Todorov Institute of Biology and Immunology of Reproduction, Sofia
  • R. Konakchieva Institute of Biology and Immunology of Reproduction, Sofia
  • J. Dimitrov University Hospital “Maichin dom”, Sofia
  • V. Novachkov University Hospital “Maichin dom”, Sofia
  • D. Kyurkchiev University Hospital “Ivan Rilski”, Sofia
  • S. I. Drokin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Gorokhova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fetal liver cells, adherent fraction, cell culture, immunophenotype analysis

Abstract

Morphology and behavior of human fetal liver cells (hFLCs) during culture were investigated. The adherent fraction of fetal liver cells was found to posses a good proliferative activity. Light end electron microscopic assesment showed the large nucleus size and the poor content of cytoplasm organelles evidenced the low differentiation status of the cell culture. In this respect immunophenotypic analysis was carried out and low expression of HLA-DR, CD13 and CD45 was determined. The data obtained by flow cytometry revealed poor content of differentiated and antigen-presenting cells in the examined cultures of hFLCs.

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Published

2006-06-15

How to Cite

Todorov, P., Konakchieva, R., Dimitrov, J., Novachkov, V., Kyurkchiev, D., Drokin, S. I., & Gorokhova, N. A. (2006). Morphological and Functional Assessment of Human Fetal Liver Cells in Long-Term Culture. Problems of Cryobiology and Cryomedicine, 16(2), 201–206. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/601

Issue

Vol. 16 No. 2 (2006): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 P. Todorov, R. Konakchieva, J. Dimitrov, V. Novachkov, D. Kyurkchiev, S. I. Drokin, N. A. Gorokhova

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