Effect of Glycerol, Ethylene Glycol on Morphofunctional Characteristics of Dogs’ Spermatozoa at Different Cryopreservation Stages

Authors

  • M. I. Egorov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. F. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. N. Kuchkov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

spermatozoa, motility, equilibration, cryopreservation, glycerol, ethylene glycol

Abstract

There has been studied the effect of glycerol and ethylene glycol (EG) on survival of dogs’ spermatozoa (DS) after freeze-thawing depending on the concentration of cryoprotectants, cooling regimens and equilibration time, as well as the change in DS cell volume after the cryoprotectants being introduced into suspension by means of turbidimetric method. The highest motility of DS was revealed after cryopreservation under EG protection with final concentration of 7% when being frozen at the first stage with the rate of 3°C/min with no preliminary equilibration. It has been shown that 7% EG causes quite less alterations in cell volume if compared to glycerol.

Downloads

Published

2004-12-15

How to Cite

Egorov, M. I., Kopeika, E. F., Linnik, T. P., & Kuchkov, I. N. (2004). Effect of Glycerol, Ethylene Glycol on Morphofunctional Characteristics of Dogs’ Spermatozoa at Different Cryopreservation Stages. Problems of Cryobiology and Cryomedicine, (4), 13–20. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/655

Issue

No. 4 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 M. I. Egorov, E. F. Kopeika, T. P. Linnik, I. N. Kuchkov

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).