Proteinases and Its Inhibitor Activity at Artificial Hypometabolic State in Rats

Authors

  • A. V. Shilo Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Lomako Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. M. Samokhina Institute of Therapy named after L.T. Malaya of the Academy of Medical Sciences of Ukraine, Kharkov
  • G. A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

artificial hypometabolic state, proteinases, inhibitors of proteinases, brain, peripheric tissues

Abstract

The proteinases, nontrypsin-like proteinases (NTLP), α-1-proteinase inhibitor ( α-1-IP) and α-2-macroglobulin (α-2-MG) activities in serum, tissues of cerebral cortex, brain stem, hypothalamus, cerebellum, lung, heart, liver and kidneys in rats under the artificial hypometabolic state (AHMS) attained by Andjus-Bakhmetiev-Giaja methods have been studied. Multidirectional changes of the indices of proteinase-inhibitor proteinase activity were revealed: total activity of proteinases slightly increased, the α-1-IP activity were stable to the influence, while the NTLP and α-2-MG activities demonstrated high sensitivity to the AHMS induced factors. Observing at AHMS the proteolysis activation mainly connected to increasing non-trypsin-like enzymes and α-2-MG level reducing in all examined tissues. In 24 hrs after AHMS the levels of the studied indices in general turned to initial levels with exception in organs which may have the maximum loading at AHMS development.

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Published

2004-06-15

How to Cite

Shilo, A. V., Lomako, V. V., Samokhina, L. M., & Babijchuk, G. A. (2004). Proteinases and Its Inhibitor Activity at Artificial Hypometabolic State in Rats. Problems of Cryobiology and Cryomedicine, (2), 17–27. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/684

Issue

No. 2 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. V. Shilo, V. V. Lomako, L. M. Samokhina, G. A. Babijchuk

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