Effect of Low Temperature Storage on Steroid Hormones Content Stability in Human Oocyte Follicular Fluid

Authors

  • E. A. Yakovleva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. G. Gerodes Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

follicular fluid of human oocyte, estradiol, progesterone, testosterone, low temperature storage

Abstract

There was studied the estradiol (E), progesterone (P) and testosterone (T) content in follicular fluid (FF) of human oocyte preovulatory follicles, native and low-temperature-stored one (-196°C). We have compared the effect of two freezing regimens (one-step immersion into liquid nitrogen and a two-step one with the preliminary exposure in nitrogen vapors) on the stability of concentration maintenance of these hormones in FF for 1 and 6 months. Stability of estradiol, progesterone and testosterone was shown to be kept within the whole observation period and to be independent upon the FL freezing regimen selected.

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Published

2004-03-15

How to Cite

Yakovleva, E. A., & Gerodes, A. G. (2004). Effect of Low Temperature Storage on Steroid Hormones Content Stability in Human Oocyte Follicular Fluid. Problems of Cryobiology and Cryomedicine, (1), 83–88. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/709

Issue

No. 1 (2004): Problems of Cryobiology

Section

Cryomedicine, Clinical and Experimental Transplantology

License

Copyright (c) 2020 E. A. Yakovleva, A. G. Gerodes

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