Ultrastructure and Biological Properties of Avian Infectious Bronchitis Virus Following Cryopreservation

Authors

  • Marina Yu. Stegniy Institute of of Experimental and Clinical Veterinary Medicine, Kharkov
  • Boris T. Stegniy Institute of of Experimental and Clinical Veterinary Medicine, Kharkov
  • Anatoliy N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo25.04.340

Keywords:

avian infectious bronchitis virus, morphology, ultrastructure, virion, cryopreservation, cryobank, cryodamage

Abstract

The authors investigated ultrastructure and infectious activity of avian infectious bronchitis virus following cryopreservation and low temperature storage at –20, –70 and –196°C during various terms from 3 days up to 8 years using electron microscopy, serologic and virusologic methods. It was shown that the lower was the storage temperature of cryopreserved viruses, the higher was the preservation of their ultrastructure and biological properties. Ultrastructure changes in IBV following long-term storage at moderate low temperatures (–20°C) included the damages of virion lipoprotein membrane, loss of glycoprotein ‘crown’ in most virions, conformation destabilization of membrane and nucleocapside. Following storage of IBV at –70 and –196°C the most virions retained electric charge and the ‘crown’ peplomers during whole observation term, damaged lipoproteid envelope and compromized structure were found only in 10% of total virions.


Probl Cryobiol Cryomed 2015; 25(4):340-349.

Author Biographies

Marina Yu. Stegniy, Institute of of Experimental and Clinical Veterinary Medicine, Kharkov

Laboratory of Biotechnology

Boris T. Stegniy, Institute of of Experimental and Clinical Veterinary Medicine, Kharkov

Laboratory of Biotechnology

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Published

2015-12-21

How to Cite

Stegniy, M. Y., Stegniy, B. T., & Goltsev, A. N. (2015). Ultrastructure and Biological Properties of Avian Infectious Bronchitis Virus Following Cryopreservation. Problems of Cryobiology and Cryomedicine, 25(4), 340–349. https://doi.org/10.15407/cryo25.04.340

Issue

Vol. 25 No. 4 (2015): Problems of Cryobiology and Cryomedicine

Section

Theoretical and Experimental Cryobiology

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Copyright (c) 2020 Marina Yu. Stegniy, Boris T. Stegniy, Anatoliy N. Goltsev

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