Problems of Cryobiology and Cryomedicine

Cover of the Issue

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Editorial Information

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2024-11-15T16:00:06+01:00

Probl Cryobiol Cryomed 2024; 34(2):70

The Modern Cryobank as Key Support for Clinical Cell and Tissue Transplantation Programmes

2024-11-15T16:04:18+01:00

The authors review the essential role of modern cryobanks in the development of cryotechnology before the start of the clinical cell and tissue transplantation programme and in the routine support of these programmes within the legislative framework settled by the European Union directives. Two clinical programmes are presented as examples: the autologous and allogeneic haematopoietic progenitor cell (HPC) transplantation programme and the Czech national cryopreserved allogeneic vascular tissue (VT) transplantation programme supported by the cryobank of the University Hospital Hradec Králové, which is a part of the European Union Tissue Establishment registered under the codes CZ000425, CZ000426 and CZ000427. An overview of necessary approvals to be obtained before the start of clinical programmes is presented, the high quality of cryopreservation technology and high level of cryobank staff safety is demonstrated, and the crucial role of the cryobank in clinical HPC and VT transplantation programmes is highlighted.

Probl Cryobiol Cryomed 2024; 34(2):71–112

Wheat Seed Germination After Low-Temperature Treatment or Ozonation

2024-11-15T17:15:07+01:00

The influence of pre-sowing treatment of Solomiya, Verden and Staleva winter wheat seeds either with ozonation or low temperatures (‒20, ‒40, ‒80 and ‒196°C) on their initial growth parameters was under study. The findings demonstrated a stimulating effect of ‒20 and ‒80°C on dynamics of seed germination and biometric parameters of seedlings for all the cultivars. The winter wheat seeds cooled down to ‒40°C showed virtually no changes in their germination rates compared to the control. Cooling down to ‒196°C worsened the germination of Solomiya seeds, decreasing the shoot length, dry weight of sprouts and roots as compared to the control. Verden and Staleva cultivars revealed the root elongation and accelerated seed germination. Ozonation caused no effect on dynamics of seed germination in all the studied wheat cultivars, except for Staleva, where some inhibition of germination was noted. According to biometric parameters, the root length increased in all the cultivars, and dry weight of sprouts was additionally augmented in Solomiya one.

Probl Cryobiol Cryomed 2024; 34(2):113–124

Impact of Bacterization with Anabaena flos-aquae and Pseudomonas putida and Salicylic Acid Treatment on Cold Resistance in Leguminous Plants

2024-11-15T17:33:01+01:00

The paper presents the results of studying the eff ects of exposure of pea (Pisum sativum) and been (Phaseolus vulgaris) seeds or young sprouts to low temperature (4°C) with preliminary treatment of seeds with salicylic acid (SA), or with pure cultures of Anabaena flos-aquae or Pseudomonas putida, or with their mixture. Cold exposure lasting 24 hrs was carried out immediately or 7 days after SA treatment or bacterization of seeds. Morphometric parameters of roots and sprouts, permeability of cell membranes in leaves, activity of polyphenol oxidase and state of actin filaments in root cells were measured. Pea and bean plants differ in cold resistance, and their growth responses to low temperature exposure were multidirectional. Treatment with SА immediately before exposure to cold had a positive effect on cold resistance of pea plants. Under the delayed cold stress, seed bacterization with single bacteria suspensions or their mixture contributed to the development of cold resistance in beans. Bacterization of seeds presumably reduced the permeability of cell membranes in leaves of both plant species and improved the state of actin filaments in root cells. In general, some protective effect for peas was observed due to either bacterial or SА treatment of seeds. In bean plants, the meaningful protective effect occurred after seed bacterization only.

Probl Cryobiol Cryomed 2024; 34(2):125–142

Pituitary-Thyroid System in Rats of Different Ages Under Short-Term Cold Exposures

2024-11-15T17:53:28+01:00

Probl Cryobiol Cryomed 2024; 34(2):143–149

To the 70th Anniversary of Ukraine’s Membership in UNESCO. UNESCO Chair in Cryobiology: Milestones, Experience, Results, Perspectives

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Probl Cryobiol Cryomed 2024; 34(2):150–157

Instruction for Authors

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Probl Cryobiol Cryomed 2024; 34(2):160–161

Backmatter

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Probl Cryobiol Cryomed 2024; 34(2): 162