Effect of Incubation of PK-15 Line Cells with Cryoprotectants on Amount of Gal-alpha-1,3-Gal Epitopes
Keywords:Gal-α-1, 3-Gal epitope, cryoprotectants, dimethyl sulfoxide, polyethylene glycol
Transplantation of bioprostheses derived from bovine and porcine tissues is an advanced method of correcting defects in blood vessels and valves, however when using it a hyperacute immune response associated with the presence of Gal-alpha- 1,3-Gal (α-Gal epitope) on the surface of cell membranes can develop. We have studied the effect of penetrating and nonpenetrating cryoprotectants on the amount of α-Gal epitopes on membranes of PK-15 cell line of porcine origin. Cell labeling with BSIB4 lectin conjugated with FITC and flow cytometry revealed a significant increase in the number of fluorescent cells after incubation with various concentrations of dimethyl sulfoxide and polyethylene glycol with MW of 1500 (PEG-1500). The revealed effect may be associated with an additional exposure of α-Gal epitopes on cell membrane, locally deformed due to the action of cryoprotectants.
Probl Cryobiol Cryomed 2016; 26(4): 331–339
Aravind S., Paul W., Vasudev S.C. et al. Polyethylene glycol (PEG) modified bovine pericardium as a biomaterial: a comparative study on immunogenicity. J Biomater Appl 1998; 13(2): 158–165. CrossRef
Badylak S. F., Gilbert T. W. Immune response to biologic scaffold materials. Semin. Immunol 2008; 20(2): 109–116. CrossRef
Belous A.M, Grischenko V.I. Cryobiology. Kyiv: Naukova Dumka; 1994.
Blackall D.P., Armstrong J.K., Meiselman H.J. et al. Polyethylene glycol-coated red blood cells fail to bind glycophorin A-specific antibodies and are impervious to invasion by the Plasmodium falciparum malaria parasite. Blood 2001; 97(2): 551–556. CrossRef PubMed
Deglon N., Aubert V., Spertini F.P. et al. Presence of Gal-alpha 1,3- Gal epitope on xenogeneic lines: implications for cellular genetherapy based on the encapsulation technology. xenotransplantation. 2003; 10(3): 204–213. CrossRef PubMed
Diaz T.M., Pertega S., Ortega D. et al. FDA/PI flow cytometry assay of complement-mediated cytotoxicity of antibodies generated during xenotransplantation. Cytometry A 2004; 62(1): 54–60. CrossRef PubMed
Ezzelarab M., Ayares D., Cooper D.K.C. Carbohydrates in xenotransplantation. Immunol Cell Biol 2005; 83(4): 396–404.
Galili U. The α-Gal epitope (Gal1-3Galα1-4GlcNAc-R) in xenotransplantation. Biochemie 2001; 83(7): 557. CrossRef
Galili U., Clark M.R., Shohet S.B. et al. Evolutionary relationship between the natural anti-Gal antibody and the Gal-α-l,3-Gal epitope in primates. Proc Natl Acad Sci USA Immunology 1987; 84(5): 1369–1373. CrossRef
Galili U., Shohet S.B., Kobrin E. et al. Man, apes, and Old World monkeys differ from other mammals in the expression of α-galactosyl epitopes on nucleated cells. J Biol Chem 1988; 263(33): 17755–17762.
Galili U., Swanson K. Evolution gene sequences suggest inactivation of α-1,3-galactosyltransferase in catarrhines after the divergence of apes from monkeys. Proc Natl Acad Sci USA 1991; 88(16): 7401–7404. CrossRef
Goldstein I.J., Blake D.A., Ebisu S. et al. Carbohydrate binding studies on the Bandeiraea simplicifolia 1 isolectins. Lectins which are mono-, di-, tri-, and tetravalent for N-acetyl-D-galactosamine. J Biol Chem 1981; 256(8): 3890–3893. PubMed
Goldstein S., Clarke D.R., Walsh S.P. et al. Transpecies heart valve transplant: advanced studies of a bioengineered xenoautograft. Ann Thorac Surg 2000; 70(6): 1962–1969. CrossRef
Gonzalez-Andrades M., de la Cruz C.J., Ionescu A.M. et al. Generation of bioengineered corneas with decellularized xenografts and human keratocytes. Invest Ophthalmol Vis Sci 2011; 52(1): 215. CrossRef PubMed
Gurtovenko A.A., Anwar J. Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide. J Phys Chem B 2007; 111(35): 10453–104560. CrossRef PubMed
Han D.K., Jeong S.Y., Kim Y.H. Evaluation of blood compatibility of PEO grafted heparin immobilized polyurethanes. J Biomed Mater Res Appl Biomater 1989; 23(A2 Suppl): 211–228. PubMed
Jarocha D., Zuba-Surma E., Majka M. Dimethyl sulfoxide (DMSO) increases percentage of CXCR4(+) hematopoietic stem/progenitor cells, their responsiveness to an SDF-1 gradient, homing capacities, and survival cell. Cell Transplant 2016; 25(7): 1247–1257. CrossRef PubMed
Kuleshova L.G. Transformation of human erythrocytes in nonelectrolytes of H-alcohols series. Part I. Morphological aspects of interaction. Problems of Cryobiology 1999; (1): 9–13.
Lehtonen J.Y., Kinnunen P.K. Poly(ethylene glycol)-induced and temperature-dependent phase separation in fluid binary phospholipid membranes. Biophys J 1995; 68(2): 525–535. CrossRef
Lim H. G., Kim G. B., Jeong S. et al. Development of a nextgeneration tissue valve using a glutaraldehyde-fixed porcine aortic valve treated with decellularization, α-galactosidase, space filler, organic solvent and detoxification. EJCTS 2015; 48(1): 104–113.
Pushkar N.S., Belous A.M., Tsvetkov Ts.D. Theory and practice of cryogenic preservation and sublimation. Kyiv: Naukova Dumka; 1984. 29.
Roy H.K., DiBaise J.K., Black J. et al. Polyethylene glycol induces apoptosis in HT-29 cells: potential mechanism for chemoprevention of colon cancer. FEBS Lett 2001; 496(2–3): 143–146. CrossRef
Simon P., Kasimir M.T., Seebacher G. et al. Early failure of the tissue engineered porcine heart valve SYNERGRAFT in pediatric patients. Eur J Cardiothorac Surg 2003; 23(6): 1002–1006. CrossRef
Wang D., Kyluik D.L., Murad K.L et al. Polymer-mediated immunocamouflage of red blood cells: effects of polymer size on antigenic and immunogenic recognition of allogeneic donor blood cells. Sci China Life Sci 2011; 54(7): 589–598. CrossRef PubMed
Wollmann L.C., Laurindo C.A., Costa F.D. et al. Effects of cryopreservation and/or decellularization on extracellular matrix of porcine valves. Rev Bras Cir Cardiovasc 2011; 26(3): 490–496. CrossRef PubMed
Zemlianskykh N. G., Babiychuk L.A. Cryopreservation in presence of PEG-1500 affects erythrocyte surface characteristics. Probl Cryobiol Cryomed 2015; 25(2): 104–113. CrossRef
Zemlianskykh N. G. Effect of substances with cryoprotective properties on surface marker CD44 in human erythrocytes. Cytology and Genetics 2016; 50(3): 203–213. CrossRef
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