Comparative Evaluation of Biological Activity of Fraction Below 5 kDa from Cattle Cord Blood After Low-Temperature Storage (at –80°C) or Lyophilization to Treat Burn Wounds in Rats
Keywords:low molecular weight fraction, cord blood, low temperature storage, lyophilization, biological activity, burn
Abstract: Biological activity of the low molecular weight (down to 5 kDa) fraction from cattle cord blood after storage in lyophilized state at 4°C and –80°C in the form of solution in a model of thermal burn of rats’ skin was investigated. Actovegin with a pronoun-ced wound healing activity was used as a comparator drug. The storage of low molecular weight fraction for 3 months under the chosen conditions was found not to reduce its biological activity. Introduced and lyophilized cord blood fraction to experimen-tal animals after simulation of thermal skin burns contributed to a reduced burn wound area, normalization of leukocyte content and phagocytic activity of neutrophils in peripheral blood. The ﬁ ndings enable considering the lyophilization of the low molecular weight fraction followed by storage at 4°C as sensibly more expedient option of storing and transporting the biologically active substance.
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