Comparative Evaluation of Biological Activity of Fraction Below 5 kDa from Cattle Cord Blood After Low-Temperature Storage (at -80°C) or Lyophilization to Treat Burn Wounds in Rats

Authors

  • Oleksandr Gulevskyy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Natalia Moiseyeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga Gorina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Andriy Nikolchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Ivan Schenyavsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.01.047

Keywords:

low molecular weight fraction, cord blood, low temperature storage, lyophilization, biological activity, burn

Abstract

Biological activity of the low molecular weight (down to 5 kDa) fraction from cattle cord blood after storage in lyophilized state at 4°C and -80°C in the form of solution in a model of thermal burn of rats' skin was investigated. Actovegin with a pronounced wound healing activity was used as a comparator drug. The storage of low molecular weight fraction for 3 months under the chosen conditions was found not to reduce its biological activity. Introduced and lyophilized cord blood fraction to experimental animals after simulation of thermal skin burns contributed to a reduced burn wound area, normalization of leukocyte content and phagocytic activity of neutrophils in peripheral blood. The findings enable considering the lyophilization of the low molecular weight fraction followed by storage at 4°C as sensibly more expedient option of storing and transporting the biologically active substance.

 

Probl Cryobiol Cryomed 2020; 30(1): 47-57

Author Biographies

Oleksandr Gulevskyy, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Natalia Moiseyeva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Olga Gorina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Andriy Nikolchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Ivan Schenyavsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

References

Agita A, Alsagaff MT. Inflammation, immunity, and hypertension. Acta Med Indones. 2017 ; 49(2): 158-65.

Belous AM, Tsvetkov DU. [Scientific grounds of the technology of sublimational preservation]. Kyiv: Naukova Dumka; 1985. 208 p. Russian.

Brock TD. Membrane filtration: a user's guide and reference manual. Berlin-New York: Springer-Verlag; 1983. 381 p.

Damien P, Allan DS. Regenerative therapy and immune modulation using umbilical cord blood-derived cells. Biol Blood Marrow Transplant. 2015; 21(9): 1545-54. CrossRef

Emami F, Vatanara A, Park EJ, et al. Drying technologies for the stability and bioavailability of biopharmaceuticals. Pharmaceutics [Internet]. 2018 [cited 2018 Aug 18]; 10(3): E131. Available from: https://www.mdpi.com/1999-4923/10/3/ 131/htm CrossRef

Fuller B, Green C, Grischenko VI. Cryopreservation for Ñell banking: current concepts at the turn of the 21st century. Problems of Cryobiology. 2003; 13(2): 62-83.

Gulevsky AK, Moiseyeva NN, Abakumova YS, et al. [Studying of biological activity of fraction less than 5 kD from cattle cord blood cryohaemolizate]. Problems of Cryobiology. 2008; 18(4): 531-4. Russian.

Gulevsky AK, Moiseyeva NN, Gorina OL. Influence of low-molecular (below 5 kDa) fraction from cord blood and actovegin on phagocytic activity of frozen-thawed neutrophils. CryoLetters. 2011; 32(2): 131-40. CrossRef

Gulevsky AK, Moisieieva NN, Gorina OL, et al. [The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro]. Ukr Biochem J. 2014; 86(6): 167-74. Russian. CrossRef

Gulevsky AK, Nikolchenko AYu, Somov AYu, et al. [Influence of the fraction below 5 kDa from cattle cord blood on respiratory activity of rat liver homogenates]. Vestnik problem biologii i mediciny. 2012; 9(1): 104-6. Russian.

Gulevsky AK, Shenyavsky II, Nikolchenko AYu. [Cord blood and its components: biological features, clinical applying, storage in cryobanks]. Kharkiv: Rider; 2017. 344 p. Russian

Jang TH, Park SC, Yang JH, et al. Cryopreservation and its clinical applications. Integr Med Res. 2017; 6(1): 12-8. CrossRef

Kozinets GI, Makarova VA. [Study of the blood system in clinical practice]. Moscow: Triada; 1998. 480 p. Russian.

Mayadas TN, Cullere X, Lowell CA. The multifaceted functions of neutrophils. Annu Rev Pathol. 2014; 9: 181-218. CrossRef

Moiseyeva NN. [The influence of a low molecular fraction (less than 5 kD) from cord blood on regeneration processes in burn wounds in rats]. World of Medicine and Biology. 2009; (3): 117-20. Russian.

Obermaier-Kusser B, Mühlbacher C, Mushack J, et al. Further evidence for a two-step model of glucose-transport regulation. Inositol phosphate-oligosaccharides regulate glucose-carrier activity. Biochem J. 1989; 261(3): 699-705. CrossRef

Osetskiy AI, Grischenko VI, Snurnikov AS, et al. Cryosublimation fractionating of biological material. Problems of Cryobiology. 2006; 16 (2): 230-40.

Rozanova OE, Serova LD, Shabalin VN. [The influence of cytotoxic and hormonal drugs on the phagocytic activity of neutrophils in patients with leukemia]. Gematologiya i transfuziologiya. 1989; 34(4): 15-20. Russian.

Rumyantseva SÐ. [Actovegin. New aspects of clinical application]. Moscow; 2002. 280 p. Russian.

Svedentsov EP, Tumanova TV, Khudyakov AN, et al. [Cryo-preservation of functionally active blood nuclear cell membranes at −80°C]. Biochemistry (Moscow) Supplement: Membrane and Cell Biology. 2008; 2(1): 19-25. Russian.

Yelsky VN, Strelchenko YuI, Zyablitzev SV. [Changes in phagocytic immunity in rats with dosed burn injury and subsequent exposure to polarized light]. Travma. 2012; 13(4): 70-4. Ukrainian.

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Published

2020-03-28

How to Cite

Gulevskyy, O., Moiseyeva, N., Gorina, O., Nikolchenko, A., & Schenyavsky, I. (2020). Comparative Evaluation of Biological Activity of Fraction Below 5 kDa from Cattle Cord Blood After Low-Temperature Storage (at -80°C) or Lyophilization to Treat Burn Wounds in Rats. Problems of Cryobiology and Cryomedicine, 30(1), 47–57. https://doi.org/10.15407/cryo30.01.047

Issue

Section

Theoretical and Experimental Cryobiology