Design of Effective Cryopreservation Conditions for Candida Yeast-Like Fungi

I. P. Vysekantsev, V. F. Martsenyuk, A. Yu. Sirenko

Abstract


The influences of freezing rates over 0.25–100°C/min range and DMSO, glycerol and ethanol cryoprotectants on the viability of yeast-like fungi C.albicans ATCC885 were studied. It has been shown that maximum level of survival of yeast-like fungi was provided by freezing rates over 3.5–10°C/min range in beer wort and adding of 5% DMSO or 5% glycerol.


Keywords


yeast-like fungi; viability; cryopreservation; cryoprotectants

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