New Fluorescent Dyes as Fluorescent Probes for Examination of Canine Spermatozoa in Cryoprotective Media

Authors

  • M. I. Еgorov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. P. Belonozhko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. G. Еrmolenko Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov
  • O. N. Semenova Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov
  • L. D. Patsenker Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov
  • V. G. Pivovarenko Taras Shevchenko Kiev National University, Kiev
  • E. A. Povrozin Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

canine spermatozoa, dimethyl formamide, ethylene glycol, fluorescent probes, DSM, E-176, 3-DAB, FME

Abstract

Possibilities of using DSM, E-176, 3-DAB, FME fluorescent dyes for examining the effect of cryoprotective agents on canine spermatozoa were studied with the methods of fluorescent spectroscopy and microscopy. FME and 3-DAB dyes were found to be capable of solving the tasks set and DSM, E-176 ones had a limitation due to quite a strong fluorescence of cryoprotectants’ solutions. Studied fluorescent dyes were demonstrated as differently affecting cell motility.

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Published

2006-03-31

How to Cite

Еgorov, M. I., Dyubko, T. S., Linnik, T. P., Belonozhko, A. P., Еrmolenko, I. G., Semenova, O. N., Patsenker, L. D., Pivovarenko, V. G., & Povrozin, E. A. (2006). New Fluorescent Dyes as Fluorescent Probes for Examination of Canine Spermatozoa in Cryoprotective Media. Problems of Cryobiology and Cryomedicine, 16(1), 13–23. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/612

Issue

Section

Theoretical and Experimental Cryobiology