New Fluorescent Dyes as Fluorescent Probes for Examination of Canine Spermatozoa in Cryoprotective Media

Authors

  • M. I. Еgorov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. S. Dyubko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. P. Belonozhko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. G. Еrmolenko Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov
  • O. N. Semenova Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov
  • L. D. Patsenker Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov
  • V. G. Pivovarenko Taras Shevchenko Kiev National University, Kiev
  • E. A. Povrozin Institute for Single Crystals of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

canine spermatozoa, dimethyl formamide, ethylene glycol, fluorescent probes, DSM, E-176, 3-DAB, FME

Abstract

Possibilities of using DSM, E-176, 3-DAB, FME fluorescent dyes for examining the effect of cryoprotective agents on canine spermatozoa were studied with the methods of fluorescent spectroscopy and microscopy. FME and 3-DAB dyes were found to be capable of solving the tasks set and DSM, E-176 ones had a limitation due to quite a strong fluorescence of cryoprotectants’ solutions. Studied fluorescent dyes were demonstrated as differently affecting cell motility.

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Published

2006-03-31

How to Cite

Еgorov, M. I., Dyubko, T. S., Linnik, T. P., Belonozhko, A. P., Еrmolenko, I. G., Semenova, O. N., Patsenker, L. D., Pivovarenko, V. G., & Povrozin, E. A. (2006). New Fluorescent Dyes as Fluorescent Probes for Examination of Canine Spermatozoa in Cryoprotective Media. Problems of Cryobiology and Cryomedicine, 16(1), 13–23. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/612

Issue

Vol. 16 No. 1 (2006): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 M. I. Еgorov, T. S. Dyubko, T. P. Linnik, A. P. Belonozhko, I. G. Еrmolenko, O. N. Semenova, L. D. Patsenker, V. G. Pivovarenko, E. A. Povrozin

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