Studying the Effect of Potato Cold Hardening on Integrity of Meristems, Cryopreserved by Slow Freezing

T. F. Stribul, N. A. Shevchenko, L. F. Rozanov

Abstract


Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in comparison with the same index in non-hardened meristems. Potato apex hardening is inexpedient when using 1,2-PD. Observation for cryopreserved meristem development within 30 days has demonstrated that treatment with cryoprotectants and freezing inhibit an apex growth in comparison with the control. 1,2-PD occurred to be less toxic. DMSO treatment can cause callus-genesis.


Keywords


meristem (apex); cold hardening; cryoprotectants; cryopreservation; freezing; integrity

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