Studying the Effect of Potato Cold Hardening on Integrity of Meristems, Cryopreserved by Slow Freezing

Authors

  • T. F. Stribul Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Shevchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

meristem (apex), cold hardening, cryoprotectants, cryopreservation, freezing, integrity

Abstract

Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in comparison with the same index in non-hardened meristems. Potato apex hardening is inexpedient when using 1,2-PD. Observation for cryopreserved meristem development within 30 days has demonstrated that treatment with cryoprotectants and freezing inhibit an apex growth in comparison with the control. 1,2-PD occurred to be less toxic. DMSO treatment can cause callus-genesis.

Published

2006-03-31

How to Cite

Stribul, T. F., Shevchenko, N. A., & Rozanov, L. F. (2006). Studying the Effect of Potato Cold Hardening on Integrity of Meristems, Cryopreserved by Slow Freezing. Problems of Cryobiology and Cryomedicine, 16(1), 60-65. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/617

Issue

Section

Theoretical and Experimental Cryobiology