Influence of Duration of a Moderate Low Temperature Storage on RNA of Cattle Diarrhea Virus Strains
Keywords:
diarrhea virus, moderate low temperatures, polymerase chain reactionAbstract
There was studied the effect of storage duration under conditions of moderate low temperature (from –18 down to –30°C) on the possibility to change nucleotide sequence of their genome. Analysis of obtained results testify to the fact that within the most conservative site (15’-untranslated region) of cattle diarrhea virus genome the nucleotide sequences are not impaired both in case of virus storage at temperatures from –18 down to –30°C for 13 months and under longer storage term (for 12 years). In reaction with outer primers there was impossible to reveal the products of polymerase chain reaction and when using inner primers with the length of 195 pairs of nucleotides they were obtained, that confirmed the RNA degradation during storage of moderate low temperatures.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 M. Yu. Stegniy, A. N. Goltsev, B. T. Stegniy
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).