Study of Devitrification Kinetics of Cryoprotectant Aqueous Solutions Using Thermoplastic Deformation Method

  • A. I. Osetsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. L. Kirilyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
Keywords: thermoplastic deformation, devitrification temperature, cryoprotectant, DMSO, PEO-1500, glycerol

Abstract

There was considered the method for investigating frozen solutions of cryoprotectants, cell suspensions and tissues, based on measuring their plastic deformation in the regimen of continuously increasing temperature within the range of -196…20°C at a constant external load. Using this method the devitrification temperatures Tg of aqueous solutions of such cryoprotectants as glycerol, DMSO, PEO-1500 within a wide range of concentrations were determined. For the first time Tg values within 5…15% (weight/weight) concentrations range were found-out.

Published
2005-06-15
How to Cite
Osetsky, A. I., Kirilyuk, A. L., & Gurina, T. M. (2005). Study of Devitrification Kinetics of Cryoprotectant Aqueous Solutions Using Thermoplastic Deformation Method. Problems of Cryobiology and Cryomedicine, 15(2), 137-146. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/626
Section
Theoretical and Experimental Cryobiology