Effect of Encapsulation into Alginate Microspheres on Viability of Mesenchymal Stromal Cells after Exposure with Penetrating Cryoprotectants

Authors

  • Vedeney S. Zaikov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Dmitriy N. Tarusin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Aleksandr Yu. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.03.213

Keywords:

mesenchymal stromal cells, alginate microspheres, vitrification, multicomponent solution of cryoprotectants, dimethyl sulfoxide, ethylene glycol, 1, 2-propane diol

Abstract

The effect of encapsulation into alginate microspheres (AMS) on viability of mesenchymal stromal cells (MSCs) after exposure to the solutions of penetrating cryoprotectants dimethyl sulfoxide (DMSO), ethylene glycol (EG) and 1,2-propane diol (1,2‑PD) with concentrations varying from 1.5 to 9 M during 1.5 and 5 min was studied. It was shown that the viability of MSCs both as a suspension and encapsulated in AMS decreased with a rise in cryoprotectant concentration and exposure time. Encapsulation into AMS significantly protected the cells from cytotoxic damage of the cryoprotectants, e. g. if the 5-min exposure to 9 M DMSO, EG and 1,2-PD resulted in the death of quite all the cells in the suspension, the viability of encapsulated MSCs after exposure under similar conditions made 60, 80 and 52% respectively. It was found that the studied penetrating cryoprotectants had a various cytotoxicity to MSCs, and it  decreased in the row DMSO > 1,2-PD > EG. The findings can be used to develop a multi-component vitrifying solution.

 

Probl Cryobiol Cryomed 2016; 26(3): 213–220

Author Biographies

Vedeney S. Zaikov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Dmitriy N. Tarusin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Aleksandr Yu. Petrenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

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Published

2016-09-23

How to Cite

Zaikov, V. S., Tarusin, D. N., & Petrenko, A. Y. (2016). Effect of Encapsulation into Alginate Microspheres on Viability of Mesenchymal Stromal Cells after Exposure with Penetrating Cryoprotectants. Problems of Cryobiology and Cryomedicine, 26(3), 213-220. https://doi.org/10.15407/cryo26.03.213

Issue

Section

Theoretical and Experimental Cryobiology