Permeability Coefficients of Murine Enterocyte Membranes for Water and Cryoprotectants
Keywords:murine enterocytes, filtration coefficient, permeability coefficient, cryoprotectants, glycerol, 1, 2-propane diol, ethylene glycol, dimethyl sulfoxide
Osmotic response of cells and transport properties of membrane are essential for cryobiological research in terms of choosing the optimal conditions to cryopreserve the specific cell type. In the present study we have found the permeability coefficients of murine enterocytes to water and such cryoprotectants as: ethylene glycol (EG), glycerol, 1,2-propanediol (1,2-PD) and dimethyl sulfoxide (DMSO). The experimental time dependencies of cell volume revealed during their contact with hypertonic solutions of cryoprotectants were fitted with numeric solutions of nonlinear equations describing this dependence in terms of linear thermodynamics of irreversible processes. The found filtration coefficients had no significant differences in cryoprotectant solutions of 1,2-PD, DMSO and glycerol ((1.42; 1.3; 1.24)x10<sup>–14</sup> m<sup>3</sup>/N·sec, respectively) and were almost twice higher in EG solution (2.4)x10<sup>–14</sup> m<sup>3</sup>/N·sec). The membranes of murine enterocytes showed the highest penetration rate for EG 4.79x10<sup>–7</sup> m/s which was probably due to its negative effect on enterocyte membranes. Permeability coefficient for 1,2-PD, DMSO and glycerol was (0.672; 0.530; 0.134)x10<sup>–7</sup> m/s, respectively. The obtained data can be used in selection of the most proper regimen of cryopreservation for these cells.
Probl Cryobiol Cryomed 2016; 26(3): 221–228
Dumont F., Marechal P.A., Gervais P. Influence of cooling rate on Saccharomyces cerevisiae destruction during freezing: unexpected viability at ultrarapid cooling rates. Cryobiology 2003; 46: 33–42. CrossRef
Gordienko O.I., Gordienko E.O., Linnik T.P., Kompaniets A.M. Mechanisms of cryoprotectant penetration through erythro-cyte membranes. Problems of Cryobiology 2002; 4: 9–15.
Gordiyenko Ye.O., Pushkar N.S. Physical basis for low temperature preservation of cell suspensions. Kyiv: Naukova dumka; 1994.
Mazur P. Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells. Cryobiology 1966; 2: 181–192. CrossRef
Morris G.J., Coulson G.E., Clarke K.-J. Freezing injury in Saccharomyces cerevisiae: the effect of growth conditions. Cryobiology 1988; 25: 471–482. CrossRef
Muldrew K., McGann L.E. Mechanisms of intracellular ice formation. Biophys J 1990; 57: 525–532. CrossRef
Ogurtsova V.V., Kovalenko S.Ye., Kovalenko I.F., Gordiyenko O.I. Determination of osmotically inactive volume of murine enterocytes. Probl Cryobiol Cryomed 2016; 26(1): 93–97. CrossRef
Sakun O.V., Kovalenko I.F., Sirenko A.Yu. et al. Membrane permeability coefficients of yeast Saccharomyces cerevisiae to water and cryoprotectants. V.N. Karazin KhNU Bull Series Biol 2008; 7(814): 140–146.
Tanghe A., Van Dijck P., Colavizza D., Thevelein J. M. Aquaporin-mediated improvement of freeze tolerance of Saccharomyces cerevisiae is restricted to rapid freezing conditions. Appl Environ Microbiol 2004; 70 (6): 3377–3382. CrossRef PubMed
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).