Morphological Features of Spleen Tissue After Introduction of Fluorouracil and Cryopreserved Cord Blood Nucleated Cells

Authors

  • Olga V. Kudokotseva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Viktoriya V. Volina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Ivan I. Lomakin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.03.249

Keywords:

spleen, immuno- and hemosuppression, fluorouracil, cryopreserved cord blood cells

Abstract

We studied the effect of administered cryopreserved nucleated cells of human cord blood (cCBNCs) on murine spleen tissue morphology under hemo- and immunosuppression induced by a single intraperitoneal injection of 5-fluorouracil (5-FU) antimetabolite of nucleic metabolism in the maximum tolerated dose. Progressing changes, typical for a damage caused by toxic substances, such as: congestion, exudation and leukocytes infiltration of spleen pulp; accumulation of macrophages either with phagocytized lymphocytes or their fragments; degenerative and necrotic changes in tissue elements of the pulp and follicles were revealed in spleen following  5‑FU introduction within entire observation period (16 days). A high degree of murine spleen reactivity in response to the administration of cryopreserved cord blood cells was found. To day 2 after cCBNCs administration we observed structural rearrangements in spleen, and already 10 days later 5-FU antimetabolite toxic injury the organ structure was back to the norm.

 

Probl Cryobiol Cryomed 2016; 26(3): 249–258

Author Biographies

Olga V. Kudokotseva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Viktoriya V. Volina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Ivan I. Lomakin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

References

Babijchuk L.A., Kudokotseva O.V., Zubov P.M., Zubova O.L. New approaches to cryopreservation of cord blood nucleated cells and assessment of their viability. Ukrainskiy Khimiotera-pevticheskiy Zhurnal 2008; (1–2): 85–88.

Babijchuk L.A., Zubov P.M., Ryazantsev V.V. et al. Cord blood as an alternative source of stem sells for regenerative medicine: new approaches to problem of cryopreservation. Buk Med Herald 2009; 13(4): 23–26.

Bobrysheva I.V. Morphological reactivity of rat spleen during different ages at immunostimulation. Journal of Clinical and Experimental Medical Research 2013; 1(3): 315–321.

Frolov V.M., Drannik G.M. Clinical immunology of increased fatigability syndrome in Donbass region residents: indices of cellular immunity. Ukr Med Almanakh 2003; 6(3): 169–172.

Goldberg E.D., Dygai A.M., Zhdanov V.V. Role of hemopoiesis-inducing microenvironment in regulation of hematopoiesis under cytostatic myelosuppressions. Tomsk: STT; 1999.

Grigorieva K.V. Morphofunctional state of immune system organs under Fludinat compound effect. Pharmacology and Drug Toxicology 2013; 35(4–5): 31–33.

Ivanova S.A. Psychoemotional stress and immunity. Siberian Herald of Psychiatry and Addiction Psychiatry 2000; (1): 31–37.

Kaschenko S.A., Zolotarevskaya M.V. Morphometric indices of rat spleen after cyclophosphan administration. Ukr Med Almanakh 2011; 9(2): 31–33.

Khaitov R.M., Pinegin B.V. Modern immunomodulators: the basic principles of their application. Immunologiya 2000; (5): 4–7.

Kryzhanovsky G.N., Magayeva S.V., Makarov S.V. Neuroimmuno-pathology. Moscow: Institute of General Pathology and Pathophysiology; 1997.

Kudokotseva O.V., Kovalenko I.F., Lomakin I.I., Babijchuk G.A. Correction of anemia resulting from 5-fluorouracil toxic effect using cryopreserved preparations of cord blood. Problems of Cryobiology and Cryomedicine 2014; 24(4): 312–312.

Kudokotseva O.V., Lomakin I.I., Babijchuk G.A. Cryopreserved cord blood products mitigate fluorouracil myelodepression in mice. Problems of Cryobiology and Cryomedicine 2015; 25(4): 359–370.

Makalysh T.P. Morphofunctional features of spleen under influence of factors of various genesis. Tavricheskyi Med Biol Vestnik 2013; 16(1): 265–269.

Mashkovsky M.D. Drugs. Moscow: Novaya Volna; 2006.

Morozova O.V. Spleen as the organ implementing relationship between circulation and hematopoiesis systems. Clinical & Experimental Pathology 2015; 14(2): 130–134.

Savin E.I., Isayeva N.M., Subbotina T.I. et al. Effect of modulating factors on formation of equilibrium conditions in terms of irreversible pathological process (experimental research). Tula: Publishing House of Tula State University; 2012.

Solovieva A.O., Poveschenko A.F., Poveschenko O.V. et al. Comparative study of migration and distribution of bone marrow and spleen donor cells into lymphoid and non-lymphoid organs of CBA mice in vitro in different terms after transplantation. Bull of SBRAMS 2013; 33(4): 35–41.

Statsenko E.A. Current concepts of human spleen anatomy. Ukr Med Almanakh 2003; 6(3): 169–172.

Li Z.H., Liao W., Cui X.L. et al. Intravenous transplantation of allogenic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head. Int J Med Sci 2011; 8(1): 74–83. CrossRef PubMed

Palumbo R., Bianchi M.E. High mobility group box 1 protein, a cue for stem cell recruitment. Biochem Pharmacol 2004; 68(6): 1165–1170. CrossRef PubMed

Tomita Y., Makino S., Hakuno D. et al. Application of mesenchymal stem cell-derived cardiomyocytes as biopacemakers: current status and problem to be solved. Med Biol Eng Comput 2007; 45(2): 209–220. CrossRef PubMed

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Published

2016-09-23

How to Cite

Kudokotseva, O. V., Volina, V. V., & Lomakin, I. I. (2016). Morphological Features of Spleen Tissue After Introduction of Fluorouracil and Cryopreserved Cord Blood Nucleated Cells. Problems of Cryobiology and Cryomedicine, 26(3), 249–259. https://doi.org/10.15407/cryo26.03.249

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology