Post-Cryo Survival of Rat Testicular Interstitial Cells in Hydroxyethyl Starch-Based Media

Authors

DOI:

https://doi.org/10.15407/cryo31.01.070

Keywords:

cryopreservation, cryoprotective agents, Leydig cells, hydroxyethyl starch

Abstract

The development of cryoprotective serum-free or xeno-free media is required for safe use of cryopreserved testicular material for transplantation. In this study, the solutions containing 10% fetal bovine serum (FBS) or 5 mg/ml bovine serum albumin (BSA) did not significantly enhance the general survival of interstitial cells (ICs) after cryopreservation but increase their metabolic activity and steroid producing cell (HSD+-cells) survival. The use of 50 and 100 mg/ml hydroxyethyl starch (HES) in DMSO-based cryoprotective solutions instead of BSA or FBS enabled the improvement of the IC general survival and the survival of HSD+-cells. The use of HES supplemented media allowed to decrease the dimethyl sulfoxide (DMSO) concentration from 1.4 to 0.7 M and to preserve the amount and metabolic activity of ICs. Thus, designing cryoprotective media containing DMSO and HES can facilitate the formulation of serum-free solutions for cryopreservation that in turn paves a way for implementation of the use of cryopreserved material for practical medicine.

Probl Cryobiol Cryomed 2021; 31(1): 070–081

Author Biography

Oleksandr V. Pakhomov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology

References

Ashwoodsmith M, Warby C, Connor K, Becker G. Low-temperature preservation of mammalian cells in tissue culture with polyvinylpyrrolidone (PVP), dextrans, and hydroxyethyl starch (HES). Cryobiology. 1972;9(5):441-9. CrossRef

Chen GR, Ge RS, Lin H, et al. Development of a cryopreservation protocol for Leydig cells. Hum Reprod. 2007;22(8):2160-8. CrossRef

Chen-Plotkin AS, Vossel KA, Samuels MA, Chen MH. Encephalopathy, stroke, and myocardial infarction with DMSO use in stem cell transplantation. Neurology. 2007;68(11):859-61. CrossRef

Connor W, Ashwood-Smith MJ. Cryoprotection of mammalian cells in tissue culture with polymers; possible mechanisms. Cryobiology. 1973;10(6):488-96. CrossRef

Dokras A, Sargent IL, Redman CW, Barlow DH. Sera from women with unexplained infertility inhibit both mouse and human embryo growth in vitro. Fertil Steril. 1993;60(2):285-92. CrossRef

Dyrlund TF, Kirkegaard K, Poulsen ET, et al. Unconditioned commercial embryo culture media contain a large variety of non-declared proteins: a comprehensive proteomics analysis. Hum Reprod. 2014;29(11):2421-30. CrossRef

Ferrucci PF, Martinoni A, Cocorocchio E, et al. Evaluation of acute toxicities associated with autologous peripheral blood progenitor cell reinfusion in patients undergoing high-dose chemotherapy. Bone Marrow Transplant. 2000;25(2):173-7. CrossRef

Gao X, Chang XB, Wu RY, Zhan BY. Allotransplantation of cryopreserved human Leydig cells. Transplant Proc. 1994;26(6):3490. PubMed

Gollub S, Schechter DC, Hirose T, Bailey CP. Use of hydroxyethyl starch solution in extensive surgical operations. Surg Gynecol Obstet. 1969;128(4):725-8. PubMed

Graham JE, Meola DM, Kini NR, Hoffman AM. Comparison of the effects of glycerol, dimethyl sulfoxide, and hydroxyethyl starch solutions for cryopreservation of avian red blood cells. Am J Vet Res. 2015;76(6):487-93. CrossRef

Gurtovenko AA, Anwar J. Modulating the structure and properties of cell membranes: the molecular mechanism of action of dimethyl sulfoxide. J Phys Chem B. 2007;111(35):10453-60. CrossRef

Hinckley GT, Johnson CJ, Jacobson KH, et al. Persistence of pathogenic prion protein during simulated wastewater treatment processes. Environ Sci Technol. 2008;42(14):5254-9. CrossRef

Imaizumi K, Nishishita N, Muramatsu M, et al. A simple and highly effective method for slow-freezing human pluripotent stem cells using dimethyl sulfoxide, hydroxyethyl starch and ethylene glycol. PLoS One [Internet]. 2014 Feb 12 [cited 2020 March 13];9(2):e88696. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088696 CrossRef

Kemmann E. Creutzfeldt-Jakob disease (CJD) and assisted reproductive technology (ART). Quantification of risks as part of informed consent. Hum Reprod. 1998;13(7):1777. CrossRef

Kenmochi T, Asano T, Maruyama M, et al. Cryopreservation of human pancreatic islets from non-heart-beating donors using hydroxyethyl starch and dimethyl sulfoxide as cryoprotectants. Cell Transplant. 2008;17(1-2):61-7. CrossRef

Keros V, Hultenby K, Borgström B, et al. Methods of cryopreservation of testicular tissue with viable spermatogonia in pre-pubertal boys undergoing gonadotoxic cancer treatment. Hum Reprod. 2007;22(5):1384-95. CrossRef

Kim H, Tanaka S, Une S, et al. A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation. J Vet Med Sci. 2004;66(12):1543-7. CrossRef

Kirpatovskii VI, Efremov GD, Frolova EV, Kudryavtseva LV. Stimulation of spermatogenesis and synthesis of testosterone by allotransplantation of neonatal testicular tissue under tunica albuginea of cryptorchid testis. Bull Exp Biol Med. 2019;166(4):497-502. CrossRef

Klinefelter GR, Hall PF, Ewing LL. Effect of luteinizing hormone deprivation in situ on steroidogenesis of rat Leydig cells purified by a multistep procedure. Biol Reprod. 1987;36(3):769-83. CrossRef

Körber C, Scheiwe MW. The cryoprotective properties of hydroxyethyl starch investigated by means of differential thermal analysis. Cryobiology. 1980;17(1):54-65. CrossRef

Leung PC, Gronow MJ, Kellow GN, et al. Serum supplement in human in vitro fertilization and embryo development. Fertil Steril. 1984;41(1):36-9. CrossRef

Lionetti FJ, Hunt SM, Mattaliano RJ, Valeri CR. In vitro studies of cryopreserved baboon granulocytes. Transfusion. 1978;18(6):685-92. CrossRef

Lionetti FJ, Hunt SM. Cryopreservation of human red cells in liquid nitrogen with hydroxyethyl starch. Cryobiology. 1975;12(2):110-8. CrossRef

Liu Y, Xu X, Ma X, et al. Cryopreservation of human bone marrow-derived mesenchymal stem cells with reduced dimethylsulfoxide and well-defined freezing solutions. Biotechnol Prog. 2010;26(6):1635-43. CrossRef

Liu Y, Xu X, Ma XH, et al. Effect of various freezing solutions on cryopreservation of mesenchymal stem cells from different animal species. Cryo Letters. 2011;32(5):425-35. PubMed

McGann LE. Differing actions of penetrating and nonpenetrating cryoprotective agents. Cryobiology. 1978;15(4):382-90. CrossRef

Persidsky M, Ellett M. Hydroxyethyl starch as a cryopreservative for nucleated mammalian-cells. Cryobiology. 1971;8(6):586-7. CrossRef

Petrenko Y. Cryopreservation of human embryonic liver cells using DMSO and high molecular weight polymers. Problems of Cryobiology. 2003; (3):80-7. Full Text

Sánchez-Salinas A, Cabañas-Perianes V, Blanquer M, et al. An automatic wash method for dimethyl sulfoxide removal in autologous hematopoietic stem cell transplantation decreases the adverse effects related to infusion. Transfusion. 2012;52(11):2382-6. CrossRef

Scheinkönig C, Kappicht S, Kolb HJ, Schleuning M. Adoption of long-term cultures to evaluate the cryoprotective potential of trehalose for freezing hematopoietic stem cells. Bone Marrow Transplant. 2004;34(6):531-6. CrossRef

Snyman E, Van der Merwe JV. Endotoxin-polluted medium in a human in vitro fertilization program. Fertil Steril. 1986;46(2):273-6. CrossRef

Stolzing A, Naaldijk Y, Fedorova V, Sethe S. Hydroxyethylstarch in cryopreservation - mechanisms, benefits and problems. Transfus Apher Sci. 2012;46(2):137-47. CrossRef

Tai J, Tze WJ, Johnson HW. Cryopreservation of rat Leydig cells for in-vitro and in-vivo studies. Hormone and Metabolic Research. 1994;26(3):145-7. CrossRef

Takahashi T, Hirsh A, Erbe E, Williams RJ. Mechanism of cryoprotection by extracellular polymeric solutes. Biophys J. 1988;54(3):509-18. CrossRef

Thomas MJ, Parry ES, Nash SG, Bell SH. A method for the cryopreservation of red blood cells using hydroxyethyl starch as a cryoprotectant. Transfus Sci. 1996;17(3):385-96. CrossRef

Truyen U, Parrish CR, Harder TC, Kaaden OR. There is nothing permanent except change. The emergence of new virus diseases. Vet Microbiol. 1995;43(2-3):103-22. CrossRef

van Os HC, Drogendijk AC, Fetter WP, et al. The influence of contamination of culture medium with hepatitis B virus on the outcome of in vitro fertilization pregnancies. Am J Obstet Gynecol. 1991;165(1):152-9. CrossRef

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Published

2021-03-30

How to Cite

Pakhomov , O. (2021). Post-Cryo Survival of Rat Testicular Interstitial Cells in Hydroxyethyl Starch-Based Media. Problems of Cryobiology and Cryomedicine, 31(1), 70–81. https://doi.org/10.15407/cryo31.01.070

Issue

Section

Cryopreservation of Biological Resources