Hypoxia as a modulating factor of human sperm capacitation and cryoresistance

Авторы

  • Maryna Petrushko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-8331-5419
  • Taisiia Yurchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; InLife Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland https://orcid.org/0000-0002-4993-9129

DOI:

https://doi.org/10.15407/cryo35.04.221

Ключевые слова:

cryopreservation, hypoxia, human spermatozoa, capacitation

Аннотация

Probl Cryobiol Cryomed. 2025; 35(4): 219—223

Биографии авторов

Maryna Petrushko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Reproductive System Cryobiology

Taisiia Yurchuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; InLife Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland

Department of Reproductive System Cryobiology

Библиографические ссылки

Agarwal A, Virk G, Ong C, et al. Effect of oxidative stress on male reproduction. World J Mens Health. 2014; 32(1): 1-17. CrossRef

Abruzzese GA, Sanchez-Rodriguez A, Roldan ERS. Sperm metabolism. Mol Reprod Dev. 2024; 91: 1-18. CrossRef

Balbach M, Ghanem L, Violante S, et al. Capacitation induces changes in metabolic pathways supporting motility of epididymal and ejaculated sperm. Front Cell Dev Biol. [Internet]. 2023 Jun 27 [cited 2025 Jun 15]; 11: 1160154. Available from: https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1160154/full CrossRef

Di Santo M, Tarozzi N, Nadalini M, et al.Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol. [Internet]. 2011 Dec 13[cited 2025 Jun 15]; 2012: 854837. Available from: https://onlinelibrary.wiley.com/doi/10.1155/2012/854837 CrossRef

Ferramosca A, Zara V. Bioenergetics of mammalian sperm capacitation. Biomed Res Int. [Internet]. 2014 Mar 25 [cited 2025 Jun 15]; 2014: 902953. Available from: https://onlinelibrary.wiley.com/doi/10.1155/2014/902953 CrossRef

González LN, Giaccagli MM, Herzfeld JD, et al. A side-by-side comparison of diff erent capacitation media in developing mouse sperm fertilizing ability. Sci Rep. [Internet]. 2024 Jun 21 [cited 2025 Jun 15]; 14(1): 14287. Available from: https://www.nature.com/articles/s41598-024-65134-w CrossRef

Hu J, Wu J, Liu X, et al. Hypoxia enhances autophagy level of human sperms. Sci Rep. [Internet]. 2024 Apr 11 [cited 2025 Jun 15]; 14: 8465. Available from: https://www.nature.com/articles/s41598-024-59213-1 CrossRef

Hungerford AJ, Harrison N, Bakos HW, et al. Development of an improved medium for the preservation of human spermatozoa. J Assist Reprod Genet. 2025; 42: 2167-80. CrossRef

Kumar H, Choi DK. Hypoxia inducible factor pathway and physiological adaptation: a cell survival pathway? Mediators Inflamm. [Internet]. 2015 Sep 27 [cited 2025 Jun 15]; 2015: 584758. Available from: https://onlinelibrary.wiley.com/doi/10.1155/2015/584758 CrossRef

Luna D, Hilario R, Dueñas-Chacón J, et al. The IMSI procedure improves laboratory and clinical outcomes without compromising the aneuploidy rate when compared to the classical ICSI procedure. Clin Med Insights Reprod Health. 2015; 9: 29-37. CrossRef

Ng KYB, Mingels R, Morgan H, et al. In vivo oxygen, temperature and pH dynamics in the female reproductive tract and their importance in human conception: a systematic review. Hum Reprod Update. 2018; 24(1): 15-34. CrossRef

Ozimic S, Ban-Frangez H, Stimpfel M. Sperm cryopreservation today: approaches, effi ciency, and pitfalls. Curr Issues Mol Biol. 2023; 45(6): 4716-34. CrossRef

Petrushko M, Yurchuk T, Todorov P, et al. New method for cryoprotectant-free freezing of human oligoasthenoteratozoospremic spermatozoa with high-molecular polymer. Cryobiology. 2021; 103: 39-44. CrossRef

Puga Molina LC, Luque GM, Balestrini PA, et al. Molecular basis of human sperm capacitation. [Internet]. Front Cell Dev Biol. 2018 Jul 27 [cited 2025 Jun 15]; 6: 72. Available from: https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2018.00072/full CrossRef

Sáez-Espinosa P, Huerta-Retamal N, Robles-Gómez L, et al. Influence of in vitro capacitation time on structural and functional human sperm parameters. Asian J Androl. 2020; 22(5): 447-53. CrossRef

Sengupta P, Pinggera GM, Calogero AE, Agarwal A. Oxidative stress affects sperm health and fertility - Time to apply facts learned at the bench to help the patient: Lessons for busy clinicians. Reprod Med Biol. [Internet]. 2024 Sep 01 [cited 2025 Jun 15]; 23(1): e12598. Available from: https://onlinelibrary.wiley.com/doi/10.1002/rmb2.12598 CrossRef

Tamburrino L, Traini G, Marcellini A, et al. Cryopreservation of human spermatozoa: functional, molecular and clinical aspects. Int J MolSci. 2023 Feb 28 [cited 2025 Jun 15]; 24(5): 4656. Available from: https://www.mdpi.com/1422-0067/24/5/4656 CrossRef

Tian Y, Chen X, Pu J, et al. Spermatogenic cell-specific type 1 hexokinase (HK1S) is essential for capacitation-associated increase in tyrosine phosphorylation and male fertility in mice. [Internet]. PLoS Genet. 2024 Jul 29 [cited 2025 Jun 15]; 20(7): e1011357. Available from: https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1011357 CrossRef

Yurchuk T, Pavlovich O, Gapon G, et al. Lipid peroxidation and DNA fragmentation in fresh and cryopreserved spermatozoa of men at different spermatogenesis state. Ukr Biochem J. 2021; 93(3): 24-9. CrossRef

Загрузки

Опубликован

2026-03-08

Как цитировать

Petrushko, M., & Yurchuk, T. (2026). Hypoxia as a modulating factor of human sperm capacitation and cryoresistance. Проблемы криобиологии и криомедицины, 35(4), 221—225. https://doi.org/10.15407/cryo35.04.221

Выпуск

Раздел

Криомедицина, клиническая и экспериментальная трансплантология