Cryopreservation of Cord Blood Nucleated Cells Using Non-Penetrating Cryoprotectant PEO-1500

Authors

  • Lyubov A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Olga A. Mykhailova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Vladimir V. Ryazantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Pavel M. Zubov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Regina K. Migunova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo26.01.024

Keywords:

hematopoietic stem/progenitor cells, nucleated cells, cord blood, cryopreservation, non-penetrating cryoprotectant, PEO-1500

Abstract

Development of wash-free cryopreservation methods for cord blood nucleated cell fraction, including the population of hematopoietic stem/progenitor cells, is an actual task for their long-term storage. Present investigation involved the technique of cord blood nucleated cells cryopreservation, comprising the isolation of cell population by the original two-step centrifugation method, the treatment with non-penetrating cryoprotectant polyethylene oxide with molecular weight of 1500 (PEO-1500) and freezing by the two-step program. This technique enabled to preserve more than 72% CD45+ and 75% CD34+ cells with the viability indices of correspondingly 83 and 91% and higher. Herewith, an increased content of reactive oxygen species (42.7 ± 6.3%) on the background of high cell number and their viability may be considered as a cells’ physiological response to the effect of cryopreservation factors.


Probl Cryobiol Cryomed 2016; 26(1):24-34.

Author Biographies

Lyubov A. Babijchuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryocytology

Olga A. Mykhailova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryocytology

Vladimir V. Ryazantsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryocytology

Pavel M. Zubov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryocytology

Regina K. Migunova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryocytology

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Published

2016-03-21

How to Cite

Babijchuk, L. A., Mykhailova, O. A., Ryazantsev, V. V., Zubov, P. M., & Migunova, R. K. (2016). Cryopreservation of Cord Blood Nucleated Cells Using Non-Penetrating Cryoprotectant PEO-1500. Problems of Cryobiology and Cryomedicine, 26(1), 24–34. https://doi.org/10.15407/cryo26.01.024

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Section

Theoretical and Experimental Cryobiology