Electric Conductivity and Resistance of Mouse Oocyte Membranes to Effect of Pulsed Electric Field in Cryoprotectant Solutions

Authors

  • Yevgeniya I. Smolyaninova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Viktor O. Shigimaga P. Vasylenko National Technical University of Agriculture, Kharkiv
  • Alla O. Kolesnikova Institute of Animal Science of National Academy of Agrarian Sciences of Ukraine, Kharkiv
  • Lyudmila I. Popivnenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Aleksandr F. Todrin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo28.04.311

Keywords:

mouse oocytes, plasma membrane, electric conductivity, cryoprotectant, electroporation, electric breakdown, pulsed electric field, intensity

Abstract

Using the method of pulsed conductometry, the values of electric conductivity of mouse oocytes in 1.0 M solutions of penetrating cryoprotectants have been first determined and the stability of their plasma membranes to the effect of pulsed electric field of increasing intensity has been investigated. For the oocytes, incubated in ethylene glycol (EG), 1,2-propanediol (1,2-PD), 2,3-butanediol (2,3-BD) and dimethyl sulfoxide (DMSO) solutions, the electric conductivity values were (23.6 ± 3.4), (21.3 ± 5.8) and (21.0 ± 2.3) and (23.6 ± 7.1) μS/cm respectively. The values of electric conductivity for mouse oocytes in acetamide (AC) and formamide (FA) solutions were (16.5 ± 6.1) and (16.9 ±± 10.7) μS/cm, respectively. Cryoprotectant solutions, belonging to alcohols (EG, 2,3-BD), as well as DMSO, were shown to have a stabilizing effect on the mouse oocyte plasma membranes under electric field action. The AC and FA solutions caused no stabilizing effect on mouse oocyte membranes, which was manifested in development of irreversible electric breakdown of plasma membranes with increasing electric field intensity.

 

Probl Cryobiol Cryomed 2018; 28(4): 311-321

Author Biographies

Yevgeniya I. Smolyaninova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Lyudmila I. Popivnenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Aleksandr F. Todrin, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

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Published

2019-03-01

How to Cite

Smolyaninova, Y. I., Shigimaga, V. O., Kolesnikova, A. O., Popivnenko, L. I., & Todrin, A. F. (2019). Electric Conductivity and Resistance of Mouse Oocyte Membranes to Effect of Pulsed Electric Field in Cryoprotectant Solutions. Problems of Cryobiology and Cryomedicine, 28(4), 311–321. https://doi.org/10.15407/cryo28.04.311

Issue

Section

Theoretical and Experimental Cryobiology