Temperature Ranges of Phase Transformations in the Cryoprotective Media Components Determined by Thermoplastic Deformation Method
Keywords:
thermoplastic deformation, fluidity limit, recrystallization, eutectic crystallization (melting), cryoprotectantAbstract
Phase transformations in multicomponent cryoprotective media and their components were investigated during freeze-thawing using the method of thermoplastic deformation. The research objects included solution of cryoprotectant DMSO (10%) and the components of cryoprotective media being often utilized as a solvents for biologicals’ cryoprotective media (distilled water, physiological saline and the culture media: medium 199, Hanks’ solution, DMEM). The paper represents the temperature ranges of crystallization (melting) of eutectic concentration mixture for DMSO solution (from –87 to –67°C), eutectic crystallization (melting) of physiological solution and the culture media (from –37 to –21°C), recrystallization before melting of bulk ice (from –21 to –15°C) and following melting of bulk ice (from –15 to –4.5°C).
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Copyright (c) 2020 T. M. Gurina, A. L. Kirilyuk
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