Antimicrobial Activity of Bifidobacteria Derivatives After Storage in a Frozen State

Authors

  • Oksana V. Knysh Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Olena Yu. Isaenko Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Evgeniy M. Babych Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Antonina M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleksandr V. Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Valentyna P. Polyanska Ukrainian Medical Stomatological Academy, Poltava
  • Svitlana V. Zachepylo Ukrainian Medical Stomatological Academy, Poltava
  • Iryna S. Danilova National Scientific Center «Institute of Experimental and Clinical Veterinary Medicine», Kharkiv

DOI:

https://doi.org/10.15407/cryo28.03.237

Keywords:

bifidobacteria, structural derivatives, metabolite derivatives, antimicrobial activity, storage, freeze-thawing

Abstract

Development of medical products of new generations which are based on probiotic derivatives requires the determination of optimal conditions to preserve the biologically active potential of the active substances, i. e. metabolites and bacterial components. The effect of storage in a frozen state of structural and metabolic derivatives of Bifidobacterium bifidumon their antimicrobial activity was investigated. Structural components were obtained by cyclic freezing-thawing of the suspension of a probiotic strain of bifidobacteria, metabolic products were derived during the cultivation of bifidobacteria in their own disintegrant. The samples containing the probiotic structural and metabolic derivatives were filtered. The antimicrobial activity of the filtrates was determined by qualitative and quantitative methods immediately after isolation and storage for 60 days at (–23 ± 1)°C and compared with the test cultures of staphylococci, corynebacteria and Pseudomonas aeruginosa. The results of the study showed that low temperature storage allowed to preserve the antimicrobial potential of filtrates: there was no difference between the antimicrobial activity of fresh filtrates and the samples after storage in a frozen state if assessed with a qualitative method. The quantitative method enabled to reveal a statistically significant decrease in the antimicrobial activity of filtrates after storage in a frozen state. The difference between the quantitative indices of the viability of test cultures after exposure in fresh and frozen-thawed filtrates was 0.2–0.8 lg CFU/ml. 

 

Probl Cryobiol Cryomed 2018; 28(3): 237–248

Author Biographies

Antonina M. Kompaniets, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryoprotectants

Oleksandr V. Pakhomov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology

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Published

2018-09-27

How to Cite

Knysh, O. V., Isaenko, O. Y., Babych, E. M., Kompaniets, A. M., Pakhomov, O. V., Polyanska, V. P., Zachepylo, S. V., & Danilova, I. S. (2018). Antimicrobial Activity of Bifidobacteria Derivatives After Storage in a Frozen State. Problems of Cryobiology and Cryomedicine, 28(3), 237–248. https://doi.org/10.15407/cryo28.03.237

Issue

Vol. 28 No. 3 (2018): Probl Cryobiol Cryomed

Section

Cryomedicine, Clinical and Experimental Transplantology

License

Copyright (c) 2018 Oksana V. Knysh, Olena Yu. Isaenko, Evgeniy M. Babych, Antonina M. Kompaniets, Oleksandr V. Pakhomov, Valentyna P. Polyanska, Svitlana V. Zachepylo, Iryna S. Danilova

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