Viability of Bifidobacterium bifidum 1 under hypothermia, single and repeated freeze-thaw cycles.
DOI:
https://doi.org/10.15407/cryo30.03.247Keywords:
viability, freeze-thaw, thermal cycling, daily biomass growth, bifidobacteria, biofilm formationAbstract
Abstract: The viability of bacteria of the Bifidobacterium bifidum 1 probiotic strain under hypothermia, single and repeated freeze-thaw cycles (thermal cycling) was studied. Samples of bifidobacterial suspensions were frozen immediately after isolation or after daily hypothermic storage in three ways to the final temperature of either (–23 ± 1) or (–196 ± 1)ºC. After slow freezing of the samples down to (–23 ± 1) ºC bigger quantitative losses of bifidobacteria were observed if compared with those after rapid freezing by a direct immersion into liquid nitrogen. Storage of the samples under hypothermia and a single freeze-thaw was accompanied with a strong inhibition of the daily growth of bifidobacteria biomass and an increased formation of biofilms. Ten-fold thermal cycling in the most unfavorable way for survival did not lead to the death of all cells in suspensions. Up to 35% of bifidobacteria remained viable. Indices of the bifidobacteria ability to enhance biomass remained at the level of 35%, and the ability to form biofilm was kept at the level of 43.7–65.5% of the corresponding indices for freshly isolated cells.
Probl Cryobiol Cryomed 2020; 30(3): 247–255
References
El-Kest SE, Marth EH. Freezing of Listeria monocytogenes and other microorganisms: a review. J Food Prot. 1992; 55(8):639-48. CrossRef
Fonseca F, Marin M, Morris GJ. Stabilization of frozen Lactobacillus delbrueckii subsp. bulgaricus in glycerol sus-pensions: freezing kinetics and storage temperature effects. Appl Environ Microbiol. 2006; 72(10):6474-82. CrossRef
Knysh OV. Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri. Regulatory Mechanisms in Biosystems. 2019; 10(1):124-8. CrossRef
Kwon YW, Bae J-H, Kim S-A, Han NS. Development of freeze-thaw tolerant Lactobacillus rhamnosus gg by adaptive laboratory evolution. Front Microbiol [Internet]. 2018 Nov 20 [cited 2020 May 15]; 9: 278 . Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2018.02781/full CrossRef
Lahtinen SJ, Gueimonde M, Ouwehand AC, et al. Probiotic bacteria may become dormant during storage. Appl Environ Microbiol. 2005; 71(3):1662-3. CrossRef
Mazur P. Freezing of living cells: mechanisms and implications. American Journal of Physiology Cell Physiology. 1984; 247(3): C125-C142. CrossRef
Novik G, Sidarenka A, Rakhuba D, Kolomiets E. Cryopreservation of bifidobacteria and bacteriophages in Belarusian collection of non-pathogenic microorganisms. Journal of Culture Collections. 2009; 6(1): 76-84.
O'Callaghan A, van Sinderen D. Bifidobacteria and their role as members of the human gut microbiota. Front Microbiol [Internet]. 2016 Jun 15 [cited 2020 May 15]; 7:925. Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2016.00925/full CrossRef
Sarkar A, Mandal S. Bifidobacteria - insight into clinical outcomes and mechanisms of its probiotic action. Microbiological Research. 2016; 192:159-71. CrossRef
Shehadul Islam M, Aryasomayajula A, Selvaganapathy PR. A review on macroscale and microscale cell lysis methods. Micromachines (Basel) [Internet]. 2017 [cited 2020 May 15]; 8(3):83. Available from: https://www.mdpi.com/2072-666X/ 8/3/83/htm CrossRef
Singh A, Vishwakarma V, Singhal B. Metabiotics: the functional metabolic signatures of probiotics: current state-of-art and future research priorities - metabiotics: probiotics effector molecules. Advances in Bioscience and Biotechnology. 2018; 9(4):147-89. CrossRef
Speranza B, Liso A, Corbo MR. Use of design of experiments to optimize the production of microbial probiotic biofilms. Peer J [Internet]. 2018 Jul 10 [cited 2020 May 15]; 6:e4826. Available from: https://peerj.com/articles/4826/ CrossRef
Suez J, Elinav E. The path towards microbiome-based metabolite treatment. Nature Microbiology [Internet]. 2017 May 25 [cited 2020 May 15]; 2: 17075. Available from: https://www. nature.com/articles/nmicrobiol201775 CrossRef
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Oksana V. Knysh, Oleksandr V. Pakhomov, Antonina M. Kompaniets, Valentina P. Polianska, Svitlana V. Zachepylo
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
