Viability of Bifidobacterium bifidum 1 under hypothermia, single and repeated freeze-thaw cycles.
DOI:
https://doi.org/10.15407/cryo30.03.247Ключевые слова:
viability, freeze-thaw, thermal cycling, daily biomass growth, bifidobacteria, biofilm formationАннотация
Abstract: The viability of bacteria of the Bifidobacterium bifidum 1 probiotic strain under hypothermia, single and repeated freeze-thaw cycles (thermal cycling) was studied. Samples of bifidobacterial suspensions were frozen immediately after isolation or after daily hypothermic storage in three ways to the final temperature of either (–23 ± 1) or (–196 ± 1)ºC. After slow freezing of the samples down to (–23 ± 1) ºC bigger quantitative losses of bifidobacteria were observed if compared with those after rapid freezing by a direct immersion into liquid nitrogen. Storage of the samples under hypothermia and a single freeze-thaw was accompanied with a strong inhibition of the daily growth of bifidobacteria biomass and an increased formation of biofilms. Ten-fold thermal cycling in the most unfavorable way for survival did not lead to the death of all cells in suspensions. Up to 35% of bifidobacteria remained viable. Indices of the bifidobacteria ability to enhance biomass remained at the level of 35%, and the ability to form biofilm was kept at the level of 43.7–65.5% of the corresponding indices for freshly isolated cells.
Probl Cryobiol Cryomed 2020; 30(3): 247–255
Библиографические ссылки
El-Kest SE, Marth EH. Freezing of Listeria monocytogenes and other microorganisms: a review. J Food Prot. 1992; 55(8):639-48. CrossRef
Fonseca F, Marin M, Morris GJ. Stabilization of frozen Lactobacillus delbrueckii subsp. bulgaricus in glycerol sus-pensions: freezing kinetics and storage temperature effects. Appl Environ Microbiol. 2006; 72(10):6474-82. CrossRef
Knysh OV. Bifidogenic properties of cell-free extracts derived from probiotic strains of Bifidobacterium bifidum and Lactobacillus reuteri. Regulatory Mechanisms in Biosystems. 2019; 10(1):124-8. CrossRef
Kwon YW, Bae J-H, Kim S-A, Han NS. Development of freeze-thaw tolerant Lactobacillus rhamnosus gg by adaptive laboratory evolution. Front Microbiol [Internet]. 2018 Nov 20 [cited 2020 May 15]; 9: 278 . Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2018.02781/full CrossRef
Lahtinen SJ, Gueimonde M, Ouwehand AC, et al. Probiotic bacteria may become dormant during storage. Appl Environ Microbiol. 2005; 71(3):1662-3. CrossRef
Mazur P. Freezing of living cells: mechanisms and implications. American Journal of Physiology Cell Physiology. 1984; 247(3): C125-C142. CrossRef
Novik G, Sidarenka A, Rakhuba D, Kolomiets E. Cryopreservation of bifidobacteria and bacteriophages in Belarusian collection of non-pathogenic microorganisms. Journal of Culture Collections. 2009; 6(1): 76-84.
O'Callaghan A, van Sinderen D. Bifidobacteria and their role as members of the human gut microbiota. Front Microbiol [Internet]. 2016 Jun 15 [cited 2020 May 15]; 7:925. Available from: https://www.frontiersin.org/articles/10.3389/fmicb.2016.00925/full CrossRef
Sarkar A, Mandal S. Bifidobacteria - insight into clinical outcomes and mechanisms of its probiotic action. Microbiological Research. 2016; 192:159-71. CrossRef
Shehadul Islam M, Aryasomayajula A, Selvaganapathy PR. A review on macroscale and microscale cell lysis methods. Micromachines (Basel) [Internet]. 2017 [cited 2020 May 15]; 8(3):83. Available from: https://www.mdpi.com/2072-666X/ 8/3/83/htm CrossRef
Singh A, Vishwakarma V, Singhal B. Metabiotics: the functional metabolic signatures of probiotics: current state-of-art and future research priorities - metabiotics: probiotics effector molecules. Advances in Bioscience and Biotechnology. 2018; 9(4):147-89. CrossRef
Speranza B, Liso A, Corbo MR. Use of design of experiments to optimize the production of microbial probiotic biofilms. Peer J [Internet]. 2018 Jul 10 [cited 2020 May 15]; 6:e4826. Available from: https://peerj.com/articles/4826/ CrossRef
Suez J, Elinav E. The path towards microbiome-based metabolite treatment. Nature Microbiology [Internet]. 2017 May 25 [cited 2020 May 15]; 2: 17075. Available from: https://www. nature.com/articles/nmicrobiol201775 CrossRef
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Copyright (c) 2020 Oksana V. Knysh, Oleksandr V. Pakhomov, Antonina M. Kompaniets, Valentina P. Polianska, Svitlana V. Zachepylo
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