Clinical Outcomes of Infertility Treatment Cycles for Repeatedly Cryopreserved Embryos
Keywords:
cryopreservation, vitrification, pre-implantation embryos, cryodamageAbstract
The study analyzed the survival, re-expansion, and implantation rates of human preimplantation embryos at the blastocyst stage following one and two rounds of cryopreservation using vitrification. Embryos subjected to repeated cryopreservation showed significantly lower survival, re-expansion, and implantation rates compared to those cryopreserved once (р < 0.05 for survival, р < 0.001 for re-expansion and implantation). The highest implantation rate was observed in the group with embryos cryopreserved once (р < 0.001). The results indicate that repeated cryopreservation negatively affects the viability and implantation of embryos, potentially due to structural and functional impairments. These findings highlight the importance of carefully assessing and considering potential risks when planning infertility treatment using repeatedly cryopreserved embryos.
Probl Cryobiol Cryomed 2024; 34(3): 236–244
References
Allen M, Hale L, Lantsberg D, et al. Post-warming embryo morphology is associated with live birth: a cohort study of single vitrified-warmed blastocyst transfer cycles. J Assist Reprod Genet. 2022;39(2):417–25. CrossRef
Buderatska N, Gontar J, Petrushko M, et al. Embryological characteristics and preimplantation genetic testing for aneuploidy of embryos derived from cryopreserved oocytes of women of different reproductive ages. Biopreserv Biobank. 2023;21(6):576–82. CrossRef
Check JH, Brittingham D, Swenson K, et al. Transfer of refrozen twice-thawed embryos do not decrease the implantation rate. Clin Exp Obstet Gynecol. 2001;28(1):14–6. PubMed
ESHRE Guideline Group on Good Practice in IVF Labs; De los Santos MJ, Apter S, et al. Revised guidelines for good practice in IVF laboratories (2015). Hum Reprod. 2016; 31(4): 685–6. CrossRef
Kovačič B, Taborin M, Vlaisavljević V, et al. To collapse or not to collapse blastocysts before vitrification? A matched case-control study on single vitrified-warmed blastocyst transfers. Reprod Biomed Online. 2022; 45(4):669–78. CrossRef
Kumasako Y, Otsu E, Utsunomiya T, et al. The efficacy of the transfer of twice frozen-thawed embryos with the vitrification method. Fertil Steril. 2009; 91(2):383–6. CrossRef
Li J, Xiong S, Zhao Y, et al. Effect of the re-vitrification of embryos at different stages on embryonic developmental potential [Internet]. Front Endocrinol (Lausanne). 2021[cited 2024 Aug 25];12:653310. Available from: https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2021.653310/full CrossRef
Pan Y, Wu R, Wang Z, et al. The effect of freezing twice during assisted reproductive technology on perinatal and neonatal outcomes [Internet]. Biomed Res Int. 2022 [cited 2024 Aug 25]; 2022: 5623462. Available from: https://onlinelibrary.wiley.com/doi/10.1155/2022/5623462 CrossRef
Petrushko M, Yurchuk T, Piniaiev V, et al. Cryopreservation of incomplete compacted morulae and preliminary biopsy of excluded fragments. Zygote. 2019; 27(6): 386–91. CrossRef
Pierson HE, Invik J, Meriano J, et al. A novel system for rapid conversion of Gardner embryo grades to linear scale numeric variables. Reprod Biomed Online. 2023; 46(5):808–18. CrossRef
Pomeroy KO, Comizzoli P, Rushing JS, et al. The ART of cryopreservation and its changing landscape. Fertil Steril. 2022;117(3):469–76. CrossRef
Telugu BP, Pence L. Development of pre-implantation mammalian blastocyst. Adv Anat Embryol Cell Biol. 2021; 234:21–40. CrossRef
Tsai S, Johal J, Malmsten J, et al. Embryo ploidy in vitrified versus fresh oocytes: Is there a difference? J Assist Reprod Genet. 2023; 40(10):24192–5. CrossRef
Vitale NJ, Myers MW, Denniston RS, et al. In-vitro development of refrozen mouse embryos. Hum Reprod. 1997; 12(2):310–6. CrossRef
Yurchuk T, Petrushko M, Fuller B. State of the art in assisted reproductive technologies for patients with advanced maternal age. Zygote. 2023; 31(2):149–56. CrossRef
Zhang Q, Yu W, Jin C, et al. Impact of multiple vitrification-warming procedures and insemination methods on pregnancy and neonatal outcomes in preimplantation genetic testing for aneuploidy. Reprod Sci. 2023; 30(7):2302–12. CrossRef
Zheng X, Chen Y, Yan J, et al. Effect of repeated cryopreservation on human embryo developmental potential. Reprod BioMed Online. 2017; 35(6):627–32. CrossRef
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