Pretreatment with Ozone Elevates the Resistance of Saccharomyces cerevisiae Yeast Membranes during Freeze-Thawing
DOI:
https://doi.org/10.15407/cryo24.01.038Keywords:
Saccharomyces cerevesiae, freeze-thawing, ozone, cross adaptation, oxidative stress, adaptive responseAbstract
Тhe effect of ozone on injury of Saccharomyces cerevisiae yeast membranes following freezing (down to –196°C) and warming (up to 30°C) was investigated using fluorescent staining and flow cytometry. It has been shown that freeze-thawing of S. cerevisiae cells in physiological solution resulted in the injury of membranes in 65.6% of total cells population. Treatment of the cells with ozone in a dose of 29–240 рmol О3/106 cells prior to freezing allowed to reduce significantly the number of damaged cells: (16.1 ± 0.9)% of total population. The observed effect was explained by cross-adaptation caused by ozone introduction: the adaptive response to slight oxidative stress initiated the genetically determined mechanisms of responces to other stresses, including the one caused by freeze-thawing.
Probl Cryobiol Cryomed 2014; 24(1):38–46.
References
Aguirela J., Randez-Gil F., Prieto J.A. Cold response in Saccharomyces cerevisiae: new functions of old mechanisms. FEMS Microbiol Rev 2007; 31(3): 327–341. CrossRef PubMed
Alexieva V., Ivanov S., Sergiev I., Karanov E. Interaction between stress. Bulg J Plant Physiol 2003; Special Issue: 1–17.
Collinson L., Dawes I. Inducibility of the response of yeast cells to peroxide stress. J Gen Microbiol 1992; 138: 329–335. CrossRef PubMed
Coote P.J., Cole M.B., Jones M.V. Induction of increased thermotolerance in Saccharomyces cerevisiae may be triggered by a mechanism involving intracellular pH. J Gen Microbiol 1991; 137(7): 1701–1708. CrossRef PubMed
Craig E.A. Chaperones: helpers along the pathways to protein folding. Science 1993; 260(5116): 1902–1903. CrossRef
Ermolenko D.N., Makhatadze G.I. Bacterial cold-shock proteins. Cell Mol Life Sci 2002; 59 (11). – P. 1902–1913.
Estruch F. Stress-controlled transcription factors, stress-induced genes and stress tolerance in budding yeast. FEMS Microbiol Rev 2000; 24(4): 469–486. CrossRef PubMed
Flattery-O'Brien J., Collinson L. P., Dawes I. W. Saccharomyces cerevisiae has an inducible response to menadione which differs from that to hydrogen peroxide. J Gen Microbiol 1993; 139(3): 501–507. CrossRef PubMed
Goryachaya I.P., Dyubko T.S., Zinchenko V.D. et al. Squaraine dye Square-460 as a marker of membrane cryodamage. Problems of Cryobiology and Cryomedicine 2013; 23(4): 347–350.
Homma T., Iwahashi H., Komatsu Y. Yeast gene expression during growth at low temperature. Cryobiology 2003; 46(3): 230–237. CrossRef
Iwahashi H., Obuchi K., Fujii S., Komatsu Y. The correlative evidence suggesting that trehalose stabilizes membrane structure in the yeast Saccharomyces cerevisiae. Cell Mol Biol 1995; 41(6): 763–769. PubMed
Jiang W., Hou Y., Inouye M. CspA, the major cold-shock protein of Escherichia coli, is an RNA chaperone. J Biol Chem 1997; 272(16): 196–202. PubMed
Kandror O., Bretschneider N., Kreydin E. et. al. Yeast adapt to near-freezing temperatures by STRE/Msn2,4-dependent induction of trehalose synthesis and certain molecular chaperones. Mol Cell 2004; 13(6): 771–781. CrossRef
Kohrer K., Domdey H. Preparation of high molecular weight RNA Meth Enzymol 1991; 194: 398–405. CrossRef
Lewis J.G., Learmonth R.P., Watson K. Induction of heat, freezing and salt tolerance by heat and salt shock in Saccharomyces cerevisiae. Microbiology 1995; 141(3): 687–694. CrossRef PubMed
Lu F., Wang Y., Bai D., Du L. Adaptive response of Saccharomyces cerevisiae to hyperosmotic and oxidative stress. Process Biochemistry 2005; 40(11): 3614–3618. CrossRef
Lunin V.V., Popovich M.P., Tkachenko S.N. Physical chemistry of ozone. Moscow: Moscow University; 1998. PubMed
Martinez-Pastor M.T., Marchler G., Schuller C. et al. The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress response element (STRE). EMBO J 1996; 15(9): 2227–2235. PubMed
Murakami Y., Yokoigawa K., Kawai H. Lipid composition of freeze-tolerant yeast, Torulaspora delbureckii, and its freeze-sensitive mutant. Appl Microbiol Biotechnol 1995; 44(2): 167–171. CrossRef
Murata Y., Homma T., Kitagawa E. et al. Genome-wide expression analysis of yeast response during exposure to 4°C. Extremophiles 2006; 10(2): 117–128. CrossRef PubMed
Ruis H., Schuller C. Strees signaling in yeast. Bioessays 1995; 17(11): 959–965. CrossRef PubMed
Thieringer H.A., Jones P.G., Inouye M. Cold shock and adaptation. Bioessays 1998; 20(1): 49–57. CrossRef
Varela J.C.S. Response of Saccharomyces cerevisiae to changes in external osmolarity. Microbiology 1996; 142(4): 721–731. CrossRef PubMed
Vigh L., Maresca B., Harwood J.L. Does the membrane's physical state control the expression of heat shock and other genes? Trends Biochem Sci 1998; 23(10): 369–374. CrossRef
Zinchenko V.D., Golota V.I., Sukhomlin E.A. et al. Laboratory equipment to use ozone technologies in biology and medicine. Problems of Cryobiology 2006; 16(2): 68–72.
Zinchenko V.D., Goryachaya I.P., Govor I.V. Bioluminometer. Patent 72111 (Ukraine), IPC7 G01N 21/76 (2006.01).
Zinchenko V.D., Goryachaya I.P., Govor I.V. Cuvette for bio- and chemiluminometers Patent 73452 (Ukraine), IPC7 G01N 23/03 (2006).
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